420220-M

Thiazovivin

≥98% (HPLC), powder, Rho kinase inhibitor, Calbiochem

• 同義詞: iPSC Induction Enhancer, Thiazovivin, N-Benzyl-2-(pyrimidin-4-ylamino)thiazole-4-carboxamide, Induced Pluripotent Stem Cells Induction Enhancer, Rho Kinase Inhibitor XII
• 經驗公式（希爾表示法）: C₁₅H₁₃N₅OS
• 分子量：: 311.36
• 分類程式碼代碼: 12352200

屬性

• product name: iPSC 诱导增强剂，Thiazovivin-Calbiochem, A cell-permeable aminothiazolo-carboxamide compound that is reported to augment SB431542 and PD0325901.
• 品質等級: 100
• 化驗: ≥98% (HPLC)
• 形狀: powder
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; protect from light
• 顏色: tan
• 溶解度: DMSO: 100 mg/mL
• 儲存溫度: 2-8°C

描述

一般說明

A cell-permeable aminothiazolo-carboxamide compound that is ~5-fold more potent than Y-27632 (Cat. Nos. 688000 & 688001) in inhibiting ROCK enzymatic activity (71% inhibition at 2 µM). Single-cell dissociation of cultured hESCs is demonstrated to hyperactivate Rho-ROCK signaling and result in cell death, while suppression of such hyperactivation by Tzv (2 µM) or Y-27632 (2 µM) stabilize newly synthesized cell surface E-cadherin, which in turn promotes cell-cell adhesion and prevents trypsinization-induced apoptosis (12% vs 81% TUNEL-positive cells after trypsinization in matrigel cultures with 2 µM Tzv or DMSO, respectively). Unlike, Ptn (Cat. No. 544049), Tzv protects dissociated individual hESCs even in the absence of ECM (24% vs 90% TUNEL-positive cells after trypsinization in non-coating plates with 2 µM Tzv or Ptn, respectively). Simultaneous blockage of TGFβ and MEK-ERK pathways by 2 µM SB431542 (Cat. No. 616461)and 0.5 µM PD0325901 (Cat. No. 444966) is reported to result in >50-fold improvement of human iPSCs (induced Pluripotent Stem Cells) generation from fibroblast cultures virally transfected with 4-TF (Oct44, Klf4, Sox2, and c-Myc), while the inclusion of 0.5 µM Tzv on top of SB431542 and PD0325901 is demonstrated to further improve the efficiency to an overall improvement of >200-fold from 4-TF-induction alone.

A cell-permeable aminothiazolo-carboxamide compound that is ~5-fold more potent than Y-27632 (Cat. Nos. 688000 & 688001) as a ROCK inhibitor (71% inhibition at 2 µM). Effectively prevents trypsinization-induced apoptosis in hESC cultures by stabilizing newly synthesized cell surface E-cadherin and cell-cell adhesion. Unlike, Ptn (Cat. No. 544049), Tzv protects dissociated hESCs even in the absence of ECM. Greatly augments SB431542 (Cat. No. 616461)and PD0325901 (Cat. No. 444966) in improving iPSCs generation efficiency from 4-TF- (Oct44, Klf4, Sox2, and c-Myc) transfected fibroblast cultures.

警告

Toxicity: Standard Handling (A)

重構

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

其他說明

Xu, Y., et al. 2010. Proc. Natl. Acad. Sci. USA107, 8129
Lin, T., et al. 2009. Nat. Methods6, 805.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable