14-451-M

ROKα/ROCK-II Protein, active, 10 µg

Active, N-terminal His6-tagged recombinant human ROCK-II residues 11-552, for use in Kinase Assays.

• 同義詞: ROK, Renal carcinoma antigen NY-REN-35, Rho kinase, Rho-associated, coiled-coil-containing protein kinase 1, p160ROCK
• 分類程式碼代碼: 12352202
• eCl@ss: 32160405
• NACRES: NA.41

屬性

• 生物源: human
• 品質等級: 100
• 重組細胞: expressed in baculovirus infected Sf21 cells
• 形狀: liquid
• 儲存期限: 6 mo.
• 分子量: M_w 63.3 kDa
• 製造商／商標名: Upstate^®
• 技術: activity assay: suitable (kinase)
• 溶解度: soluble
• NCBI登錄號: NM_005406.2
• UniProt登錄號: Q13464
• 儲存溫度: −70°C
• 基因資訊: human ... ROCK1(6093)

描述

一般說明

Research area: APOPTOSIS

N-terminal His6-tagged recombinant human ROCK-II residues 11-552
Product Source: expressed by baculovirus in Sf21 insect cells

Rho kinase (ROCK) is a type of serine/threonine kinase. There are two isoforms, ROCK1 and ROCK2. In humans ROCK1 and ROCK2 are composed of 33 exons and located on chromosome 18 (18q11.1). The ROCK protein structure is characterized by a catalytic kinase domain and Rho-binding domain (RBD) at N-terminal and pleckstrin-homology (PH) domain and an internal cysteine-rich (CR) domain at C-terminal.

生化／生理作用

Protein Target: ROKα/ROCK-II
Target Sub-Family: AGC

ROCK (Rho kinase) is activated by interacting with Rho GTPase enzyme. ROCK plays a crucial role in various essential cellular functions like adhesion, contraction, migration, and proliferation. Moreover, it regulates the cytoskeleton and actomyosin contractility during apoptosis. Overexpression of ROCK protein has been observed in various types of cancer such as osteosarcoma, hepatocellular carcinomas, colon and bladder cancers.

包裝

Also available in 250μg size (14-451M), please inquire for pricing and availability.

品質

routinely evaluated by phosphorylation of the Long S6 Kinase substrate peptide

外觀

Ni²⁺/NTA-agarose

儲存和穩定性

6 months at -70°C

其他說明

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.