1.09293

KOVACS′ indole reagent

for detecting microbial indole in the identification of indole-positive and indole-negative microorganisms, suitable for microbiology

• 同義詞: 4-(Dimethylamino)benzaldehyde solution, Indole reagent, Kovac′s reagent, Kovac′s reagent for indoles
• 分類程式碼代碼: 41106212
• NACRES: NA.21

屬性

• 品質等級: 200
• 形狀: liquid
• 包裝: pkg of 100 mL
• 技術: microbe id | specific enzyme detection: suitable
• 顏色: yellow
• pH值: <1 (20 °C in H₂O)
• 轉變溫度: flash point 36 °C
• 密度: 0.92 g/cm³ at 20 °C
• 應用: microbiology
• 儲存溫度: 2-8°C
• 適合性: Escherichia coli; enterovirulent E. coli (EEC) spp.

描述

一般說明

Kovac′s reagent is a biochemical reagent composed of n-Butanol, hydrochloric acid, and 4-dimethylaminobenzaldehyde. It is used for the detection of indole producing microorganisms. Tryptophan is abundant in trypticalle digested peptone. Some microorganisms can cleave tryptophan to give pyruvic acid, ammonia, and indole. Indole then reacts with 4-dimethylaminobenzaldehyde to form a dark red dye. As tryptophan also gives a color reaction with 4 dimethylaminobenzaldehyde, it must be separated from the indole. This is achieved by selectively extracting indole with butanol.

應用

Kovac′s Indole reagent is used for the detection of indole producing microorganisms in food and beverages, and environmental samples. This is suitable for the identification of E. coli.

儲存和穩定性

No metal containers. Keep the container tightly closed in a dry and well-ventilated place. Keep away from heat and sources of ignition

分析報告

Indole formation (Escherichia coli ATCC 25922 (WDCM 00013)): +
Indole formation (Proteus vulgaris ATCC 8427): +
Indole formation (Enterobacter aerogenes ATCC 13048): -
Tested in DEV-Tryptophan broth art. no. 1.10694. (after incubation 24 hrs.; 35 °C aerobic).

其他說明

The reagent solution must be stored in the dark in the refrigerator, otherwise it may turn brown and cannot be used.

安全資訊

• 象形圖: GHS02,GHS05,GHS07
• 訊號詞: Danger
• 危險聲明: H226 - H290 - H302 - H314 - H317 - H335 - H336
• 防範說明: P210 - P233 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• 危險分類: Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1 - Skin Sens. 1
• 儲存類別代碼: 3 - Flammable liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: 96.8 °F
• 閃點（°C）: 36 °C