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一般說明
革兰氏染色试剂盒用于鉴别性染色技术,在所有微生物实验室中应用最广泛。它是所有类型细菌分离物鉴定方案中最重要的标准之一。革兰氏阳性菌和革兰氏阴性菌细胞壁之间存在许多生理差异。自从克里斯蒂安·革兰发明革兰氏染色后,这一过程就被广泛研究和重新定义。 在实践中,将细菌细胞的薄涂片用结晶紫染色,然后用含碘媒染剂处理以增加主要染色剂的结合力。使用酒精或丙酮的脱色溶液去除结合力较弱的细胞中的结晶紫,然后用复染剂番红精为脱色的细胞带来颜色对比。在通过革兰氏染色程序正确染色的涂片中,革兰氏阳性菌呈蓝色至紫色,革兰氏阴性细胞呈粉红色至红色。
應用
革兰氏染色法是微生物学中最重要的染色技术之一。它也是用于鉴别革兰氏阳性菌和革兰氏阴性菌的所有类型细菌分离物的任何鉴定方案中的最重要标准。
成分
试剂盒含有(每个 250ml):
94448革兰氏′结晶紫溶液
90107革兰氏′碘溶液
75482革兰氏′脱色剂溶液
94635革兰氏′番红精溶液
94448革兰氏′结晶紫溶液
90107革兰氏′碘溶液
75482革兰氏′脱色剂溶液
94635革兰氏′番红精溶液
訊號詞
Danger
危險分類
Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3
標靶器官
Central nervous system
安全危害
儲存類別代碼
3 - Flammable liquids
閃點(°F)
68.0 °F
閃點(°C)
20 °C
個人防護裝備
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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