04-1501

Anti-B7-H3 (CD276) Antibody, clone MJ8

clone MJ8, from rat

• 同義詞: B7 homolog 3, CD276 antigen, CD276 molecule, Costimulatory molecule
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• 無性繁殖: MJ8, monoclonal
• application: FACS; IHC; WB
• 物種活性: human
• 技術: flow cytometry: suitable; immunohistochemistry: suitable; western blot: suitable

屬性

• 生物源: rat
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: MJ8, monoclonal
• 物種活性: human
• 物種活性（以同源性預測）: mouse (based on 100% sequence homology)
• 技術: flow cytometry: suitable; immunohistochemistry: suitable; western blot: suitable
• 同型: IgG2bκ
• NCBI登錄號: NP_598744.1
• UniProt登錄號: Q8VE98
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... CD276(80381)

描述

一般說明

B7 homolog 3 (B7-H3 or CD276) is a costimulatory molecule belonging to the B7 family that signals through the CD28 family of molecules, including CD28, CTLA4 and ICOS. B7-H3 may be involved in the regulation of T-cell-mediated immune response and may play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis, as well as a role of marker for detection of neuroblastoma cells. B7-H3 may also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling. B7-H3 is ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes, and it is weakly expressed in resting monocytes. B7-H3 is expressed in dendritic cells derived from monocytes, epithelial cells of sinonasal tissue, extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta.

特異性

This antibody recognizes the extracellular domain of B7-H3 (CD276).

免疫原

Epitope: Extracellular Domain

Mouse B7-H3 Ig2a FC fusion protein, consisting of the extracellular domain linked to the Fc portion of mouse IgG2a.

應用

Detect B7-H3 (CD276) using this Anti-B7-H3 (CD276) Antibody, clone MJ8 validated for use in WB, FC, IH.

Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC (Osamu, N., et al., 2008).

Immunochistochemistry Analysis: A previous lot was used by an independent laboratory in IH (Osamu, N., et al., 2008).

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

品質

Evaluated by Western Blot in human placenta tissue lysate.

Western Blot Analysis: 0.25 µg/mL of this antibody detected BN-H3 on 10 µg of human placenta tissue lysate.

標靶描述

~31 kDa observed molecular weight on human lysates corresponds to isoform 2 of B7-H3.

外觀

Protein G Purified

Format: Purified

Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
Human placenta tissue lysate

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable