03-0156-00

SMC^® Human IL-13 High Sensitivity Immunoassay Kit

for quantification of IL-13 in human serum and plasma.

• 同義詞: IL-13 Single Molecule Counting Kit, IL-13 ultra-sensitive immunoassay, Interleukin-13 immunoassay kit, NC30 immunoassay kit
• 分類程式碼代碼: 12161503
• eCl@ss: 32161000
• NACRES: NA.84

屬性

• 描述: LLOQ range: 0.02-0.08 pg/mL, refer to CoA forlot-specific LLOQ
• 物種活性: guinea pig, nonhuman primates, rat, canine
• 製造商／商標名: SMC^®
• 技術: ELISA: suitable; single molecule counting: suitable
• assay range: inter-assay cv: <20%; intra-assay cv: <15%; (Intra-assay CV n=20; Inter-assay n=5)

描述

一般說明

Interleukin 13 (IL-13) is secreted by a number of cells including T helper cells, CD4 cells and a various white blood cells and is thought to be a mediator of allergic inflammation in many tissues via the induction of IgE synthesis by B cells. Although primarily associated with the induction of airway disease, IL-13 has also been shown to inhibit the production of proinflammatory cytokines by monocytes and macrophages suggesting it has important anti-inflammatory properties.

The SMC^® Human IL-13 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna and SMCxPRO immunoassay systems detect analytes using Single Molecule Counting (SMC) technology.

特異性

Although IL-13 was detectable in non-human primate, rat, canine, and guinea pig samples, the assay has not been validated for these species.

應用

Research Category
Inflammation & Immunology

Research Sub Category
Inflammatory Disease

Immune Response

Autoimmune disease

The sensitivity of the Single Molecule Counting (SMC^®) technology gives the SMC Human IL-13 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IL-13 in human serum and plasma.

This new streamline kit has been improved with the following features while the kit performance is consistent with the legacy IL-13 v2 kit, #03-0109-02:
• Improved options for sample prep and incubation
• Lyophilization of the standard provides the kit at one storage condition
• Overnight incubation can be used
• Increased reagent volumes to accommodate automation
• Validated to run on both SMCxPRO^® and Erenna

成分

Assay Buffer

Human IL-13 Beads

Standard Diluent

Human IL-13 Detection Antibody

Human IL-13 Standard

10X Wash Buffer

Buffer D

Elution Buffer B

規格

Minimum of 5 serum samples spiked with average recovery of 90% with a range of 86-102% and 5 plasma samples spiked with average recovery of 92% with a range of 87-96%.

法律資訊

SMC is a registered trademark of Singulex

SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 象形圖: GHS06,GHS08
• 訊號詞: Danger
• 危險聲明: H302 + H332,H311,H319,H360FD,H412
• 防範說明: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P308 + P313
• 危險分類: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B
• 儲存類別代碼: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects