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760749

Sigma-Aldrich

二苯并环辛基-PEG4-生物素结合物

for Copper-free Click Chemistry

同義詞:

DBCO-PEG4-生物素

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About This Item

經驗公式（希爾表示法）:
C39H51N5O8S
CAS號碼:
1255942-07-4
分子量：:
749.92
MDL號碼:
MFCD22572661
分類程式碼代碼:
12352003
PubChem物質ID:
329766893
NACRES:
NA.22

品質等級

100

形狀

solid

反應適用性

reaction type: click chemistry

儲存溫度

−20°C

SMILES 字串

O=C(CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@H]12)NCCOCCOCCOCCOCCC(=O)NCCC(=O)N3Cc4ccccc4C#Cc5ccccc35

InChI

1S/C39H51N5O8S/c45-35(12-6-5-11-34-38-32(28-53-34)42-39(48)43-38)41-18-20-50-22-24-52-26-25-51-23-21-49-19-16-36(46)40-17-15-37(47)44-27-31-9-2-1-7-29(31)13-14-30-8-3-4-10-33(30)44/h1-4,7-10,32,34,38H,5-6,11-12,15-28H2,(H,40,46)(H,41,45)(H2,42,43,48)/t32-,34-,38-/m0/s1

InChI 密鑰

LNHSQAOQVNHUGL-QRBHCBQLSA-N

相關類別

一般說明

这种氮杂二苯并环辛-生物素衍生物是一种通用的生物素化试剂，用于标记含有叠氮化物的分子或化合物。环辛烯在促进无铜叠氮炔环加成反应中是有用的。这种二苯并环辛炔将与叠氮官能化化合物或生物分子反应，而不需要 Cu (I) 催化剂产生稳定的三唑键。后续的标记分子可以使用链霉亲和素或亲和素亲和试剂进行纯化。PEG 间隔基使整体化合物的亲水性更强，并允许与叠氮化物的反应站点和生物素检测基团之间的距离更大。

應用

采用 Synple 自动合成平台素（SYNPLE-SC002）自动标记生物素

特點和優勢

更高的温度和过多的生物素试剂会导致更有效的标记。典型反应时间长达 4 小时，但如果浓度较低或观察到低标记，则应使用更长的孵育时间。当首先溶解在水溶性有机溶剂中，然后根据需要在水性反应缓冲液中稀释时，产品最有效。DBCO-PEG4-生物素可溶于高达 0.35 mM 的水性缓冲液。

其他說明

2024年CiteAb奖，授予在帕金森研究方面取得成功的供应商

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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分析證明 (COA)

Lot/Batch Number
MKCQ2524
MKCP2830
MKCP2827
MKCN1219
MKCK2546

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Ning, X., et al.
Angewandte Chemie (International Edition in English), 47, 2253-2255 null
Andrew Vila et al.
Chemical research in toxicology, 21(2), 432-444 (2008-02-01)
Polyunsaturated fatty acids (PUFA) are primary targets of free radical damage during oxidative stress. Diffusible electrophilic alpha,beta-unsaturated aldehydes, such as 4-hydroxynonenal (HNE), have been shown to modify proteins that mediate cell signaling (e.g., IKK and Keap1) and alter gene expression
Guillaume Charron et al.
Journal of the American Chemical Society, 131(13), 4967-4975 (2009-03-14)
Fatty-acylation of proteins in eukaryotes is associated with many fundamental cellular processes but has been challenging to study due to limited tools for rapid and robust detection of protein fatty-acylation in cells. The development of azido-fatty acids enabled the nonradioactive
Daniela C Dieterich et al.
Nature protocols, 2(3), 532-540 (2007-04-05)
A major aim of proteomics is the identification of proteins in a given proteome at a given metabolic state. This protocol describes the step-by-step labeling, purification and detection of newly synthesized proteins in mammalian cells using the non-canonical amino acid
Nicholas J Agard et al.
ACS chemical biology, 1(10), 644-648 (2006-12-19)
Detection of metabolites and post-translational modifications can be achieved using the azide as a bioorthogonal chemical reporter. Once introduced into target biomolecules, either metabolically or through chemical modification, the azide can be tagged with probes using one of three highly
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文章

Copper-Free Click Chemistry

Copper-free click chemistry is an alternative approach to click chemistry that proceeds at a lower activation barrier and is free of cytotoxic transition metal catalysts.

Copper-Free Click Chemistry

Copper-free click chemistry is an alternative approach to click chemistry that proceeds at a lower activation barrier and is free of cytotoxic transition metal catalysts.

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