SRE0093

β-葡萄糖醛酸酶 来源于帽贝 (Patella vulgata)

aqueous solution

• 同義詞: β-D-葡萄糖苷酸葡萄糖醛酸水解酶
• CAS號碼: 9001-45-0
• 酶委員會編號: 3.2.1.31 (BRENDA, IUBMB)
• MDL號碼: MFCD00130629
• 分類程式碼代碼: 12352204
• NACRES: NA.54

屬性

• 重組細胞: expressed in proprietary host
• 品質等級: 300
• 形狀: aqueous solution
• 濃度: 100000-200000 units/mL
• 運輸包裝: ambient
• 儲存溫度: 2-8°C

描述

應用

比较不同酶性能的新技术文章
了解更多关于 Sigma R&D 生成的最新应用数据 使用不同来源的酶优化不同药物类别的水解，并使用色谱纯化酶降低酯酶活性的影响，导致 6-MAM 转化为吗啡

据报道，来自欧洲帽贝的酶比来自盖罩大蜗牛、牛肝脏和大肠杆菌的酶能更有效地水解阿片肽-葡糖苷酸

More on the suitability of this product for hydrolysis of different drug-glucuronide substrates is available at this page.

Poster presentation comparing the hydrolysis efficiency of novel beta-glucuronidase enzymes from recombinant limpet and recombinant E. coli sources, click at the following poster link: Hydrolysis Efficiency Evaluation of Novel Recombinant Limpet and E. coli Beta-Glucuronidase Enzymes

特點和優勢

Recombinantly expressed and purified to eliminate MAM esterase activity (6-Monoacetylmorphine→morphine). Representative analysis at high enzyme titer (50 un/μL β-glucuronidase) was less than 1% conversion of 6-MAM to morphine after 4 hours at 60 °C.

單位定義

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay).

外觀

Supplied as a solution in 100mM potassium acetate pH 5.2. It is free of detergents, carbohydrates or any other components that may interfere with sample preparation and analysis.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable