Isotope-based analysis of modified tRNA nucleosides correlates modification density with translational efficiency.

Isotope-based analysis of modified tRNA nucleosides correlates modification density with translational efficiency.

Angewandte Chemie (International ed. in English) (2012-10-06)

Caterina Brandmayr, Mirko Wagner, Tobias Brückl, Daniel Globisch, David Pearson, Andrea Christa Kneuttinger, Veronika Reiter, Antje Hienzsch, Susanne Koch, Ines Thoma, Peter Thumbs, Stylianos Michalakis, Markus Müller, Martin Biel, Thomas Carell

PMID23037940

摘要

Useful diversity: Quantification of modified tRNA nucleobases in different murine and porcine tissues reveals a tissue-specific overall modification content. The modification content correlates with rates of protein synthesis in vitro, suggesting a direct link between tRNA modification levels and tissue-specific translational efficiency.

材料

货号

品牌

产品描述

R4875

Sigma-Aldrich

核糖核酸酶A 来源于牛胰腺, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein

R6513

Sigma-Aldrich

核糖核酸酶A 来源于牛胰腺, for molecular biology, ≥70 Kunitz units/mg protein, lyophilized

R5125

Sigma-Aldrich