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一般說明
RNase A（核糖核酸酶A）是一种内切核糖核酸酶，在嘧啶核苷酸后裂解单链RNA的磷酸二酯键。它可切割3′磷酸基末端（例如，pG-pG-pC-pA-pG将切割为pG-pG-pCp 和A-pG）。对单链RNA表现出最高活性。RNase A是含有四个二硫键的单链多肽。它与RNase B不同，并非糖蛋白。核糖核酸酶不会水解DNA，因为DNA缺乏形成环状中间体所必需的2′-OH基团。RNase A还可以水解蛋白质样品中的RNA。RNase A可被His12和His119的烷基化抑制并被钾盐和钠盐活化。RNAse在重金属离子存在时受到抑制。此外，RNase也被DNA竞争性抑制。
應用
- RNase A用于去除DNA质粒和基因组DNA制品和蛋白质样品中的RNA。
- RNase A还用于RNA序列分析和保护测定。
- RNase A已用作计算辅助药物设计的工具。
- RNase A为RNA序列分析提供支持。
- RNase A水解蛋白质样品中的RNA。
- RNase A为DNA纯化提供支持。
特點和優勢
我们高度稳定的核糖核酸酶A——RNase A，适合于RNA去除、RNA测序和DNA纯化。
單位定義
1 U corresponds to the amount of enzyme which hydrolyzes the RNA at a rate constant k = 1 at 25°C and pH 5.0 (Kunitz-units); M. Kunitz, J. Biol. Chem. 164, 563 (1946)
分析報告
蛋白测定方法：E.
其他說明
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The enzyme only hydrolyzes phosphodiester linkages which originate from pyrimidine-3′-phosphates, as well as a large number of 2′,3′-cyclo compounds of cytidine and uridine derivatives; The return of pancreatic ribonucleases
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
[On the mechanism of the ribonuclease reaction. 1. The function of the pyrimidine base in the reaction].
H G Gassen et al.
European journal of biochemistry, 1(1), 36-45 (1967-03-01)
S A Benner et al.
Trends in biochemical sciences, 14(10), 396-397 (1989-10-01)
A decade after losing favor as an 'uninteresting' digestive enzyme, pancreatic ribonuclease has been found to be homologous to a series of extracellular proteins that may influence tumor cell growth, neurological development and biological differentiation. One surprising outcome of these
Influence of thyroid state on cardiac and renal capillary density and glomerular morphology in rats.
Isabel Rodríguez-Gómez et al.
The Journal of endocrinology, 216(1), 43-51 (2012-10-11)
The purpose was to analyse the cardiac and renal capillary density and glomerular morphology resulting from a chronic excess or deficiency of thyroid hormones (THs) in rats. We performed histopathological, morphometrical and immunohistochemical analyses in hypothyroid and hyperthyroid rats to
Yong Zhai et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 14(2), 386-393 (2013-01-03)
FT-IR spectroscopic and thermodynamic measurements were designed to explore the effect of a macromolecular crowder, dextran, on the temperature and pressure-dependent phase diagram of the protein Ribonuclease A (RNase A), and we compare the experimental data with approximate theoretical predictions
Nethaji Thiyagarajan et al.
Nature communications, 3, 1121-1121 (2012-10-11)
Mutations in angiogenin (ANG), a member of the ribonuclease A superfamily, are associated with amyotrophic lateral sclerosis (ALS; sporadic and familial) and Parkinson's disease. We have previously shown that ANG is expressed in neurons during neuro-ectodermal differentiation, and that it
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