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等級
for molecular biology
品質等級
用途
kit sufficient for >100 standard transformations
技術
transformation: suitable
運輸包裝
dry ice
儲存溫度
−20°C
一般說明
Sigma 的酵母转化试剂盒包含了通过醋酸锂方法高效转化酵母的所有必要试剂和对照品。
應用
适用于任何酵母菌株的转化。 方便灵活灵敏，只需 10 ng 的 DNA 即可获得阳性转化子；最佳效率在 0.1-3μg 范围。
特點和優勢
- 简单易用
- 只需 10 ng 质粒 DNA
- 任何酵母菌株的灵活性
- 足以进行超过 100 次标准转换
成分
酵母转化试剂盒包含：
- 转化缓冲液；100 ml；100 mM 醋酸锂、10 mM Tris HCl (pH 7.6) 和 1 mM EDTA
- 平板缓冲液；100 mL；40%PEG，100 mM 醋酸锂，10 mM Tris HCl，pH 7.5，1 mM EDTA
- 鲑鱼试验的脱氧核糖核酸，10 mg/mL；2 x 1 mL
- 携带 ura 基因的对照酵母质粒 DNA pRS316；10 μg
- 不含尿嘧啶的酵母合成脱落培养基补充剂；1 g
原則
用补充突变基因的质粒转化使转化子在缺乏所需成分的培养基上生长。通过在缓冲醋酸锂溶液中孵育，使酵母细胞具有转化能力。然后在含聚乙二醇 (PEG) 的溶液中将细胞与转化 DNA 和载体 DNA 一起孵育进行转化。
相關產品
产品编号
说明
价格
儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
Improved method for high efficiency transformation of intact yeast cells.
D Gietz et al.
Nucleic acids research, 20(6), 1425-1425 (1992-03-25)
Tim R Blower et al.
Nucleic acids research, 47(15), 8163-8179 (2019-07-10)
Type II topoisomerases catalyze essential DNA transactions and are proven drug targets. Drug discrimination by prokaryotic and eukaryotic topoisomerases is vital to therapeutic utility, but is poorly understood. We developed a next-generation sequencing (NGS) approach to identify drug-resistance mutations in
Mechanistic and structural insights into the regioselectivity of an acyl-CoA fatty acid desaturase via directed molecular evolution.
Vanhercke T
The Journal of Biological Chemistry, 286(15), 12860-12869 (2011)
Widening the pH activity profile of a fungal laccase by directed evolution.
Torres-Salas P
Chembiochem, 14(8), 934-937 (2013)
A simple and efficient procedure for transformation of yeasts.
R Elble
BioTechniques, 13(1), 18-20 (1992-07-01)
商品
Transformation is the process by which exogenous DNA is introduced into a cell, resulting in a heritable change or genetic modification. This was first reported in Streptococcus pneumoniae by Griffith in 1928. Transforming principle of DNA was demonstrated by Avery et al. in 1944.
实验方案
The selection of plasmids in yeast is based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (an amino acid, purine, or pyrimidine)
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