跳转至内容
Merck

Y0626

Sigma-Aldrich

不含氨基酸的酵母氮源基

Yeast classification medium used for selecting yeasts based on amino acid and carbohydrate requirements

别名:

ynb without amino acids

登录查看公司和协议定价


About This Item

分類程式碼代碼:
41106212
NACRES:
NA.85

等級

for molecular biology

品質等級

無菌

non-sterile

形狀

powder

技術

microbe id | utilization test: suitable
microbiological culture: suitable

pH值

5.2-5.6(0.67% solution)

應用

food and beverages
microbiology

儲存溫度

room temp

適合性

nonselective for Candida spp.
nonselective for Pichia spp.
nonselective for Saccharomyces spp.
nonselective for Zygosaccharomyces spp.
yeasts

一般說明

酵母氮基是培养野生型酵母公认的理想选择。目前可确知,氨基酸存在会妨碍选择性标记物使用。因此,这种培养基制剂之中不含氨基酸,可用于酵母人工染色体的克隆和操作。
酵母菌氮基是酵母菌生长的重要培养基。 这种富含营养的微生物肉汤含有氮、维生素、微量元素和盐。

應用

不含氨基酸的酵母氮基已用于:
  • 合成脂解耶氏酵母培养和鉴定所用的完全补充混合物 (SC)-Leu培养基
  • 用作酵母尿嘧啶营养性生长的选择性液体培养基组分
  • 评估酿酒酵母菌株在不同的营养条件下的转化效率

适用于根据氨基酸和碳的需要对酵母菌进行分类。 可能需要添加氨基酸和碳源(通常是葡萄糖)。

成分

组成部分,含量(μg/L,除非注明)

氮源:
硫酸铵,5.0 g/L

维生素:
生物素,2.0
泛酸钙,400
叶酸,2.0
肌醇,2.0 mg / L
烟酸,400
对氨基苯甲酸,200
盐酸吡哆醇,400
核黄素,200
盐酸硫胺素,400

微量元素:
硼酸,500
硫酸铜,40
碘化钾,100
氯化铁,200
硫酸锰,400
钼酸钠,200
硫酸锌,400

盐类:
磷酸二氢钾,1.0g/L
硫酸镁,0.5g/L
氯化钠,0.1g/L
氯化钙,0.1g/L

準備報告

1.将6.7 g酵母氮碱、5 g葡萄糖或等量的其他碳水化合物和5-10 mg所需氨基酸溶解在100 ml蒸馏水中,制备10x储备溶液。
2.必要时可加温溶解,然后过滤灭菌。
2-8°C储存10x储备溶液。使用前,在无菌条件下用无菌蒸馏水1:10稀释。

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

没有发现合适的版本?

如果您需要特殊版本,可通过批号或批次号查找具体证书。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

Simultaneous saccharification and fermentation of cellulose in ionic liquid for efficient production of alpha-ketoglutaric acid by Yarrowia lipolytica
Ryu S, et al.
Applied Microbiology and Biotechnology, 99(10), 4237-4244 (2015)
YAC Protocols, 11-11 (2006)
T Fernandes et al.
Journal of applied microbiology, 127(3), 845-855 (2019-06-19)
The aim was to isolate, identify and characterize yeasts present in rumen fluid and to select strains showing potential as probiotics. Rumen fluid was sampled from 4 herds of dairy and beef cattle and 77 yeast isolates were identified. Initial
Zi-xuan Wang et al.
Methods in molecular biology (Clifton, N.J.), 332, 115-127 (2006-08-02)
CXCR4, the receptor for stromal cell-derived factor (SDF)-1, was expressed in Saccharomyces cerevisiae, coupled to the pheromone response pathway via a chimeric Galpha subunit. Engagement of CXCR4 by SDF-1 resulted in expression of reporter genes, HIS3 or lacZ, under the
R Daniel Gietz et al.
Nature protocols, 2(1), 38-41 (2007-04-03)
Here, we describe a Library screen transformation protocol using the lithium acetate/single-stranded carrier DNA/PEG method of transformation for Saccharomyces cerevisiae. This method is suitable for screening complex plasmid libraries such as those used for yeast two-hybrid analysis. This procedure takes

商品

Technical Article on yeast media. Yeasts are eukaryotic microorganisms whose genomes have been comprehensively studied and some have been sequenced.

Traditional methods are based morphology, staining methods, enzyme reactions (metabolism) and diverse media.

Traditional methods are based morphology, staining methods, enzyme reactions (metabolism) and diverse media.

实验方案

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Yeast cultures can be grown, maintained, and stored in liquid media or on agar plates using techniques similar to those for bacterial cultures.

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门