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應用
由于在缺乏特定培养基成分（比如氨基酸、嘌呤或嘧啶）的情况下无法生长，营养缺陷型突变菌株的使用很大程度上大决定了酵母质粒的选择。 用含有突变基因的质粒转化，可使转化菌株能够在缺乏所需成分的培养基上生长。 Sigma 公司的′s 酵母营养缺陷型培养基补充剂提供了一种更丰富的培养基，可以提高产量和生长速度，并增加筛选文库或进行基因敲除时转化的成功率。
成分
除非另有说明，否则补充剂包含以下所有成分：
氨基酸：除亮氨酸外的所有标准氨基酸。所有氨基酸的浓度均为 76mg/L。
其他营养物质：腺嘌呤（18 mg/L），肌醇（76 mg/L），对氨基苯甲酸（8 mg/L），尿嘧啶（76 mg / L）。
氨基酸：除亮氨酸外的所有标准氨基酸。所有氨基酸的浓度均为 76mg/L。
其他营养物质：腺嘌呤（18 mg/L），肌醇（76 mg/L），对氨基苯甲酸（8 mg/L），尿嘧啶（76 mg / L）。
其他說明
氨基酸和其他营养素的混合物加入到无氨基酸酵母氮源。
數量
1 l 培养基将补充 1.6 g。
儲存類別代碼
13 - Non Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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实验方案
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