推荐产品
生物源
mouse
品質等級
共軛
unconjugated
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
Mab1, monoclonal
形狀
buffered aqueous solution
分子量
antigen ~100 kDa
物種活性
human
技術
microarray: suitable
western blot: 1-2 μg/mL using A231 human kidney cells
同型
IgG1
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... TOP1(7150)
一般說明
Monoclonal Anti-Topoisomerase I (mouse IgG1 isotype) is derived from the Mab1 hybridoma produced by the fusion of SP2/0 mouse myeloma cells and splenocytes from BALB/c mice immunized with human purified topoisomerase I. The protein family of DNA topoisomerases is divided into two types (type I and II). The type I enzymes include eukaryotic and bacterial topoisomerase I and III. Human DNA Topoisomerase I belong to the subtype IB enzymes. The protein has four distinct domains. There are four nuclear localization signals in the N-terminal domain. A linker domain of 77 amino acids follows the core domain. The fourth domain, which is 53 amino acids long, contains the active site Tyr723. Topoisomerase I (TOP1) gene is located on human chromosome 20q12.
免疫原
purified human topoisomerase I
應用
Monoclonal Anti-Topoisomerase I antibody produced in mouse has been used in western blot analysis.
生化/生理作用
DNA topoisomerases are enzymes that control the amount of supercoiling in DNA. Without topoisomerases, DNA cannot replicate normally. Type I enzymes of DNA topoisomerases introduce transient single strand breaks in DNA. Recently it was reported that DNA topoisomerase I is involved in modulation of RNA and is present in retroviral particles, like human immunodeficiency virus (HIV1). Furthermore, the enzyme enhances HIV-1 cDNA production in reverse transcription assays in vitro. Topoisomerase I (TOP1) serves as swivel at the time of replication, transcription and recombination, which helps in releasing over winding of duplex DNA.
外觀
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
未找到合适的产品?
试试我们的产品选型工具.
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
nwg
閃點(°F)
Not applicable
閃點(°C)
Not applicable
Mechanisms for defining supercoiling set point of DNA gyrase orthologs I. A nonconserved acidic C-terminal tail modulates Escherichia coli gyrase activity
The Journal of Biological Chemistry, 287(22), 18636-18644 (2012)
Amygdalin inhibits genes related to cell cycle in SNU-C4 human colon cancer cells
World Journal of Gastroenterology, 11(33), 5156-5156 (2005)
DNA topoisomerase II and its growing repertoire of biological functions
Nature Reviews. Cancer, 9(5), 327-327 (2009)
Tanshinone IIA potentiates the efficacy of 5-FU in Colo205 colon cancer cells in vivo through downregulation of P-gp and LC3-II.
Experimental and Therapeutic Medicine, 3(3), 555-559 (2012)
Experimental and therapeutic medicine, 3(3), 555-559 (2012-09-13)
Traditional Chinese herbal medicines are widely accepted as an option for the treatment of colorectal cancers. Danshen (Salviae miltiorrhizae Radix) is widely prescribed in traditional Chinese medicine for cardiovascular diseases. Tanshinone IIA (Tan-IIA) is extracted from Danshen. Our previous studies
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门