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Merck

T0565

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine

TMB membrane substrate, chromogenic, liquid

别名:

TMB membrane substrate

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About This Item

分類程式碼代碼:
12352204
NACRES:
NA.83

product name

用于膜的3,3′,5,5′-四甲基联苯胺(TMB)液体底物系统, ready to use solution

品質等級

形狀

liquid

儲存溫度

2-8°C

一般說明

3,3′,5,5′-四甲基联苯胺(TMB)是辣根过氧化物酶(HRP)偶联物的显色底物。它会产生永久性不可溶解的深蓝色反应产物,可应用于比色定量。

應用

3,3′,5,5′-四甲基联苯胺(TMB)显色液(转印膜用底物)可作为蛋白质免疫印迹分析(western blot)的显色底物。也可用作酶联免疫吸附测定(ELISA)的底物溶液。
3,3′,5,5′-四甲基联苯胺(TMB)细胞膜的液体底物体系:
  • 用于酶联免疫斑点法(ELISPOT)分析
  • 用作IgG过氧化物酶的底物
  • 应用于免疫复合物的可视化

外觀

即用型。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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商品

The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the ability to accurately determine these protein amounts remains a challenge. Fortunately, over the past decade, the field of proteomics has witnessed significant advances in the area of mass spectrometry. These advances, both in instrumentation and methodology, are providing researchers with sensitive assays for both identification and quantification of proteins within complex samples. This discussion will highlight some of these methodologies, namely the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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