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T8665

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine

peroxidase substrate, chromogenic, liquid

别名:

TMB

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About This Item

分類程式碼代碼:
12352204
NACRES:
NA.83

产品名称

3,3′,5,5′-四甲基联苯胺 (TMB) 液体底物体系, peroxidase substrate

品質等級

300

形狀

liquid

儲存條件

protect from light

運輸包裝

wet ice

儲存溫度

2-8°C

相关类别

應用

TMB是辣根过氧化物酶的底物。其可形成可溶性蓝色终产物，并可在370nm或655nm下读数。可以用0.5M H2SO4停止TMB反应（产生黄色溶液），并在450nm处读取数值。推荐用于ELISA（微孔）分析，不建议用于膜应用。
通过将测量的底物吸收值超过吸收最大值，3,3′,5,5′-四甲基联苯胺已在研究中用于证明，任何免疫酶测定的测量范围都可以被显著延长，而又不损害测定特征。3,3′,5,5′-四甲基联苯胺也已用于研究中，以了解具有过氧化物酶样活性的CuS凹面上层结构的形成。

注意

该底物对光非常敏感，应避免阳光或紫外光源直射。

外觀

即用型。

其他說明

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

訊號詞

Danger

危險分類

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Irrit. 2 - Flam. Liq. 3 - STOT SE 1

標靶器官

Eyes,Central nervous system

水污染物質分類（WGK）

WGK 2

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLCJ8626
SLCH1771
SLCD0040
SLCC5061
SLCB5343

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Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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