推荐产品
生物源
human
重組細胞
expressed in CHO cells
化驗
≥95% (HPLC)
≥95% (SDS-PAGE)
形狀
lyophilized
分子量
24.4 kDa
包裝
pkg of 20 μg
雜質
endotoxin, tested
UniProt登錄號
運輸包裝
wet ice
儲存溫度
−20°C
基因資訊
human ... RSPO2(340419)
相关类别
一般說明
Recombinant human R-Spondin-2 (R-Spo2) is a 24.4kDa protein consisting of 212 amino acid residues. R-Spo2 is encoded by the gene mapped to human chromosome 8q23.1. The encoded protein belongs to the R-spondin family of secreted proteins. Members of RSpo family contain two furin-like cysteine-rich domains at N- terminal end and thrombospondin structural domain and a basic charged C- terminal domain.
生化／生理作用
R-Spondin 2 (R-Spo2) plays a vital role in the activation of canonical Wnt signaling pathway, which is essential for normal morphogenesis of the respiratory tract and limbs. R-Spo2 positively regulates Wnt-dependent mineralization of osteoblasts, myogenic differentiation and hypertrophic myotube formation. R-Spo2 is also involved in skeletal development. Aberration in the expression of RSPO2 causes ossification of the posterior longitudinal ligament of the spine (OPLL), resulted by the altered differentiation of spinal ligament MSCs (mesenchymal stem cells) into chondrocytes. R-Spo2 is associated with various developmental processes, such as myogenesis, craniofacial morphogenesis, keratinocyte proliferation, osteoblast maturation, lung and limb development. R-Spo2 interacts with leucine-rich-repeat-containing G-protein-coupled receptor 5 (Lgr5) and stimulates acetylcholine receptor (AChR) clustering at the neuromuscular junction (NMJ).
外觀
Lyophilized from 10 mM Sodium Phosphate, pH 7.5 + 150 mM NaCl.
重構
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-0.5 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
R-spondin 2 promotes acetylcholine receptor clustering at the neuromuscular junction via Lgr5.
Nakashima H
Scientific Reports, 6:28512 (2016)
Secreted factor R-Spondin 2 is involved in refinement of patterning of the mammalian cochlea.
Mulvaney JF
Developmental Dynamics, 242(2), 179-188 (2013)
R-spondin 2 is required for normal laryngeal-tracheal, lung and limb morphogenesis.
Bell SM
Development, 135(6), 1049-1058 (2008)
R-Spondin family members regulate the Wnt pathway by a common mechanism.
Kim KA
Molecular Biology of the Cell, 19(6), 2588-2596 (2008)
Masahiro Nakajima et al.
American journal of human genetics, 99(1), 202-207 (2016-07-05)
Ossification of the posterior longitudinal ligament of the spine (OPLL) is a common spinal disorder that results from ectopic ossification of the posterior longitudinal ligament and causes intractable myelopathy and radiculopathy. In a previous genome-wide association study (GWAS), we found
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