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生物源
human
重組細胞
expressed in CHO cells
化驗
≥95% (HPLC)
≥95% (SDS-PAGE)
形狀
lyophilized
效力
1.0-3.0 μg/mL ED50
分子量
26.0-40.0 kDa
包裝
pkg of 20 μg
雜質
<0.1 EU/μg endotoxin, tested
顏色
white
UniProt登錄號
運輸包裝
wet ice
儲存溫度
−20°C
基因資訊
human ... RSPO1(284654)
一般說明
R-Spondin-1 (Rspo-1) 属于 Wnt 调节剂 (Rspo) 家族。目前，(Rspo) 家族由 4 个结构相关的分泌型配体 (Rspo 1-4) 组成，均含有弗林蛋白酶样和凝血酶敏感蛋白结构域。Rspo-1 在发育中的中枢神经系统的某些区域，以及肾上腺、卵巢、睾丸、甲状腺和气管中表达。重组人 R-Spondin-1 是由 243 个氨基酸残基组成的 26.7 kDa 蛋白。由于糖基化，在还原条件下，通过 SDS PAGE 分析，反应蛋白-1 以大约 40.0kDa 的表观分子量迁移。
生化／生理作用
Rspo (R-Spondin) 可与 Frizzled/LRP6（LDL 受体相关蛋白 6）受体复合物相互作用，刺激 Wnt/β-连环蛋白信号通路。Rspo-1 基因的破坏引起隐性综合征伴 XX 性反转、掌跖角化过度和易患皮肤鳞状细胞癌。Rspo-1 还与肿瘤细胞的抗辐射能力有关。
外觀
从 10 mM 磷酸钠 (pH 7.5) 冻干。
重構
打开前离心小瓶。用水复溶至 0.1-1.0 mg/mL 浓度。切勿涡旋。该溶液可在 2-8°C 条件下至多储存 1 周。为了延长储存时间，建议在含有载体蛋白（例如 0.1%BSA）的缓冲液中进一步稀释，并在-20°C 至-80°C 下储存。
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
R-Spondin proteins: a novel link to beta-catenin activation.
Kim KA, et al.
Cell Cycle, 5, 23-26 (2006)
R-spondin1 is essential in sex determination, skin differentiation and malignancy.
Parma P, et al.
Nature Genetics, 38, 1304-1309 (2006)
Xuefeng Gu et al.
Oncotarget, 6(12), 9756-9765 (2015-04-14)
Although radiation therapy is the most effective postoperative adjuvant treatment, it does not substantially improve the long-term outcomes of glioma patients because of the characteristic radioresistance of glioma. We found that R-Spondin1 (Rspo1) expression was elevated in high-grade gliomas and
Minke E Binnerts et al.
Proceedings of the National Academy of Sciences of the United States of America, 104(37), 14700-14705 (2007-09-07)
The R-Spondin (RSpo) family of secreted proteins act as potent activators of the Wnt/beta-catenin signaling pathway. We have previously shown that RSpo proteins can induce proliferative effects on the gastrointestinal epithelium in mice. Here we provide a mechanism whereby RSpo1
Yong-Guo Zhang et al.
Autophagy, 15(11), 1935-1953 (2019-03-22)
Extracellular HMGB1 (high mobility group box 1) is considered as a damage-associated molecular pattern protein. However, little is known about its intracellular role. We studied the mechanism whereby intestinal epithelial HMGB1 contributes to host defense, using cell culture, colonoids, conditional
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