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生物源
human
重組細胞
expressed in E. coli
化驗
≥80% (SDS-PAGE)
形狀
aqueous solution
分子量
59 kDa
包裝
pkg of 20 μg
製造商／商標名
Sigma-Aldrich
儲存條件
avoid repeated freeze/thaw cycles
濃度
1.8 mg/mL
技術
cell based assay: suitable
溶解度
water: soluble
NCBI登錄號
UniProt登錄號
應用
life science and biopharma
運輸包裝
dry ice
儲存溫度
−70°C
基因資訊
human ... PKM(5315)
一般說明
Research area: Cell Signaling
Pyruvate kinase (PK) has four isozymes such as PKL, PKR, PKM1, and PKM2. PKM1/2 is located in the distal convoluted tubule of the kidney, embryo, lungs, proliferative tissues, retina, adipose tissues, and islets. The PKM1 gene is located on the human chromosome at 15q23.
Pyruvate kinase (PK) has four isozymes such as PKL, PKR, PKM1, and PKM2. PKM1/2 is located in the distal convoluted tubule of the kidney, embryo, lungs, proliferative tissues, retina, adipose tissues, and islets. The PKM1 gene is located on the human chromosome at 15q23.
應用
PKM1active human has been used in:
- cell-based kinase assay to study the ability of a compound to inhibit the kinase activity of PKM1.
- oxidative stress experiments on PKM2.
- Co-immunoprecipitation (Co-IP) to determine pyruvate kinase M2 (PKM2) as a novel direct binding partner of phosphoserine aminotransferase 1 (PSAT1).
生化／生理作用
Pyruvate kinase (PK) catalyzes the dephosphorylation of phosphoenolpyruvate(PEP) into pyruvate. It is also involved in glycolysis by releasing ATP.Allosteric effects and intracellular signal transduction regulate the enzymaticactivity of PKM1. PKM1 is involved in several biological processes such asmetabolic reprogramming, and innate and acquired immune response. Itparticipates in protein interactions, protein synthesis, glycolysis, andintranuclear signal transduction.
訊號詞
Danger
危險聲明
危險分類
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
儲存類別代碼
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
从最新的版本中选择一种：
C Parkison et al.
Biochemical and biophysical research communications, 179(1), 668-674 (1991-08-30)
Using a T7 expression system, the monomer of rat pituitary pyruvate kinase, subtype M1 (PKM1), was overexpressed in Escherichia coli and purified to homogeneity. The monomeric p58-M1 has intrinsic enzymatic activity with a Vmax of 79 +/- 20 units/mg and
Rumeysa Biyik-Sit et al.
Cancers, 13(16) (2021-08-28)
An elevated expression of phosphoserine aminotransferase 1 (PSAT1) has been observed in multiple tumor types and is associated with poorer clinical outcomes. Although PSAT1 is postulated to promote tumor growth through its enzymatic function within the serine synthesis pathway (SSP)
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