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生物源
human
重組細胞
expressed in FreeStyle™ 293-F cells
化驗
≥90% (SDS-PAGE)
形狀
aqueous solution
比活性
≥3900 pmol/min-μg
分子量
36 kDa
技術
inhibition assay: suitable
適合性
suitable for molecular biology
NCBI登錄號
應用
life science and biopharma
運輸包裝
dry ice
儲存溫度
−70°C
基因資訊
human ... CTSL(1514)
一般說明
人组织蛋白酶B（基因库登录号：NM_001912），氨基酸18-333，带C-末端HIS标签，MW = 36 kDa，在FreeStyle 293-F细胞中表达。
應用
活性人组织蛋白酶L可用于激活DPP1（组织蛋白酶C）。 活性人组织蛋白酶L已用于一项研究中，该研究确定N-酰基和N-磺酰恶唑烷-2，4-二酮是丝氨酸蛋白酶的假不可逆抑制剂。 活性人组织蛋白酶L也已用于研究人衰老过程中血清中组织蛋白酶B和L活性的作用。
生化／生理作用
Cathepsin L is a cysteine protease, involved primarily in protein breakdown in the lysosome. Cathepsin L degrades nuclear transcription factors and may affect cell cycle regulation. Cathepsin L is also involved in the immune system, specifically in degrading the invariant chain during major histocompatibility complex (MHC) class II processing, a crucial step in antigen presentation. Its expression in the thymus has been demonstrated to be vital for the development of natural killer cells. Additionally, Cathepsin L contributes to recycling processes during axon outgrowth and synapse formation in the developing postnatal central nervous system. Proteases are associated with the development and progression of cancer. Therefore, cathepsin L may be a potential therapeutic target in cancer treatment.
單位定義
一个单位定义为在37℃下每分钟裂解1 pmol底物的酶量
外觀
在50 mM MES，400 mM NaCl，pH 5.0和10%甘油中配制。
準備報告
冰上解冻。 首次解冻后，短暂旋转含有酶的试管，以回收试管中的全部内容物。将酶分装成一次性使用的等分试样。 将剩余的未稀释酶分装保存于-70℃。注：酶对冻融循环非常敏感。
法律資訊
FreeStyle is a trademark of Invitrogen Corp.
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
Ricardo A Orbegozo-Medina et al.
PloS one, 14(2), e0211035-e0211035 (2019-02-02)
Recombinant proteins expressed in E. coli are frequently purified by immobilized metal affinity chromatography (IMAC). By means of this technique, tagged proteins containing a polyhistidine sequence can be obtained up to 95% pure in a single step, but some host
Jana Ilgová et al.
Molecular and biochemical parasitology, 235, 111248-111248 (2019-12-25)
The gills of the common carp, whose mucosal surface belongs to the key defence mechanisms of piscine immunity, can be infested with both the larval and adult stage of Eudiplozoon nipponicum (Monogenea). Although on their own, monogeneans do not considerably
Elda A Treviño et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 37(2), 490-502 (2018-11-21)
The etiology of joint tissue degeneration following rotator cuff tear remains unclear. Thus, the purpose of this study was to understand the timeline of protease activity in the soft tissues of the shoulder (tendon, muscle, and cartilage) that may lead
Aleksandra Wyczałkowska-Tomasik et al.
Archives of gerontology and geriatrics, 55(3), 735-738 (2012-06-15)
Cathepsin B and L are active in several physiological processes that occur in the human body. Their synthesis and activity is detectable in several tissues and processes, including skin hyperkeratosis, insemination, and embryonic development. Proteolytic enzymes are very important cellular
Ana Bela Santana et al.
Bioorganic & medicinal chemistry letters, 22(12), 3993-3997 (2012-05-19)
The synthesis, inhibitory activity and mode of action of oxazolidine-2,4-diones against porcine pancreatic elastase, here used as a model for human neutrophil elastase, are reported. The nature of N-substitution at the oxazolidine-2,4-dione scaffold has large effect on the inhibitory potency
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