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生物源
human
重組細胞
expressed in E. coli
化驗
≥60% (SDS-PAGE)
形狀
aqueous solution
分子量
86 kDa
包裝
pkg of 50 μg
儲存條件
avoid repeated freeze/thaw cycles
濃度
>0.02 mg/mL
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−70°C
基因資訊
human ... PRMT3(10196)
一般說明
Human PRMT3, GenBank Accession No. NM_005788, amino acids 2-end, with N-terminal GST tag, MW = 86 kDa, expressed in an E. coli expression system.
應用
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
單位定義
One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.
外觀
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.
準備報告
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Communications biology, 4(1), 109-109 (2021-01-27)
Protein arginine methyltransferase 3 (PRMT3) regulates protein functions by introducing asymmetric dimethylation marks at the arginine residues in proteins. However, very little is known about the interaction partners of PRMT3 and their functional outcomes. Using yeast-two hybrid screening, we identified
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