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一般說明
锚定寡核苷酸(dT)23引物用于启动聚(A)尾的mRNA，以进行cDNA合成。引物有23个胸腺嘧啶残基，在3′端有一个G、C或A残基（锚点）。这个锚点可确保寡核苷酸(dT)引物在信使的起点结合，并且没有很长的不可用序列区域。增强型AMV逆转录酶、模板RNA、引物的最佳浓度和扩增参数取决于所使用的系统，并且应该根据经验确定。
應用
锚定寡核苷酸(dT)23已用于实时定量聚合酶链反应（PCR）的cDNA合成。
锚定寡核苷酸23 引物由23个胸腺嘧啶核苷残基组成，并在3′端具有一个G、C或A残基（锚）。该锚定位点可确保寡核苷酸(dT)23引物与mRNA起始端结合，直接跳过无用的长序列区域。以poly(A)+ RNA为模板制备cDNA时，该锚定寡核苷酸 (dT)23 引物比标准寡核苷酸 (dT) 引物更具优势。
特點和優勢
- 在制备cDNA文库过程中，当序列信息不完整或缺失或者特异性引物不合适时，可以使用Oligo(dT)23锚定引物和随机九聚体（产品编号R 7647）。
- 这些引物可以在cDNA第一链合成、cDNA文库构建和各种其他应用中作为逆转录引物的替代品。
- 转录后，oligo(dT)23锚定引物在高温（高达65 °C）下启动引物的能力降低，避免干扰PCR。
- 由poly(A)+ RNA产生cDNA时，oligo(dT)23锚定引物优于oligo(dT)标准引物。
其他說明
仅供实验室使用。不可用于药物、家庭或其他用途。
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves
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商品
One approach to the analysis of gene expression is to measure the concentration of mRNA of a gene. There are several challenges to such analyses, such as the differences in half life between different transcripts, the temporal patterns of transcription and the lack of correlation between mRNA and protein.
实验方案
The 3’/5’ integrity assay is a potential first step in the identification of RNA degradation. The assay is particularly useful when a large number of samples are to be analyzed or when the degradation is less than that detected by capillary systems but still sufficient to effect qPCR analyses.
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