推荐产品
一般說明
應用
特點和優勢
- 以特定比例包含随机和寡聚 (dT) 引物，以确保 cDNA 产品中所有转录本序列的最佳表达。
- 高重复性预混液消除了其他需要成分混合的试剂盒固有的可变性
- RNase H(+) Moloney 鼠白血病病毒 (MMLV) 逆转录酶确保残余 RNA 不干扰 PCR 扩增
- 可在广泛的模板起始量范围下逆转录罕见和丰富基因
- 方便的 5X 预混液配方，快速简便的反应设置；只需添加 RNA 模板
- 方便的 5X 预混液配方，快速简便的反应设置；只需添加 RNA 模板
- 包括随机因子和oligodT引物的专有混合，以及Moloney鼠白血病病毒(MMLV)逆转录酶的RNAse H+衍生物
- 可在40分钟内无偏差、准确地将RNA转化为cDNA
- 在一次20μL的反应中可以使用1μg的RNA；反应也可以按比例放大
- 高度稳定；-20°C下最长保存1年；4°C下最长保存一个月
成分
其他說明
法律資訊
推薦
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
商品
Ago RIP to Isolate microRNA and their Targets Using Imprint® RNA Immunoprecipitation Kit
One approach to the analysis of gene expression is to measure the concentration of mRNA of a gene. There are several challenges to such analyses, such as the differences in half life between different transcripts, the temporal patterns of transcription and the lack of correlation between mRNA and protein.
实验方案
Method for reverse transcription of RNA into DNA. Uses a premixed reagent that contains reverse transcriptase, dNTPs, primers, RNase inhibitor and buffer. Fast generation of cDNA.
reverse transcription is the analysis of gene expression to measure concentration mrna a gene. there are several challenges such analyses, as differences in halflife between different transcripts, temporal patterns and lack correlation protein.
相关内容
Quantitative PCR (qPCR) or quantitative Real-Time PCR applications, utilizing fluorescent report molecules, suitable for your microRNA, genotyping, and gene expression analyses.
Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.
Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.
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