推荐产品
等級
for molecular biology
品質等級
無菌
0.2 μm filtered
形狀
liquid
成份
Dextrose, 3.603 g/L
KCl, 0.186 g/L
MgSO4, 4.8 g/L
Tryptone, 20 g/L
Yeast extract, 5 g/L
技術
microbiological culture: suitable
應用
agriculture
儲存溫度
2-8°C
適合性
nonselective for Escherichia coli
nonselective for coliforms
一般說明
含有分解代谢抑制物的培养基（SOC）是一种富含葡萄糖的微生物生长培养基，主要用于大肠杆菌感受态细胞转化后的复苏。 在这种营养丰富的微生物培养基中培养大肠杆菌细胞，可以抑制克隆载体上携带的lac启动子的意外激活，从而提高含有毒蛋白基因的重组质粒的转化效率。SOC培养基采用低盐配方，含有多肽、氨基酸、水溶性维生素和葡萄糖。
應用
SOC培养基可用于大肠杆菌DH5α 感受态细胞的转化。 它还被用于培养克隆后的白色菌落，以鉴定感兴趣的重组蛋白。
特點和優勢
SOC液体提供：
- 经过滤灭菌的即用型培养基
- 便利的包装规格
- 标准配方
相關產品
产品编号
说明
价格
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 1-1 (1989)
S Zhao et al.
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Meg L Flanagan et al.
Methods in molecular biology (Clifton, N.J.), 378, 33-52 (2008-07-09)
As a source of recombinant antigen, soluble constant fragment (Fc) fusion proteins have become valuable reagents for immunotherapy and laboratory investigations. Additional applications for these reagents include flow cytometry, immunohistochemistry, and in vitro activity assays. To aid investigators in the
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Despite insights gained through experimental models, the set of bacterial genes important for human infection is unclear for many of our most threatening pathogens. Klebsiella pneumoniae is a leading cause of health care-associated infections (HAIs) and commonly colonizes hospitalized patients
商品
General protocols for growth of competent cells and their transformation (uptake of DNA).
实验方案
Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.
General protocols for growth of competent cells in microbial medium.
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