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B9146

Sigma-Aldrich

5-溴-4-氯-3-吲哚基-β-吡咯-D-半乳糖苷

≥98%, powder, suitable for identification of lac+ bacterial colonies

别名:

5-溴-4-氯-3-吲哚基-β-吡咯-D-半乳糖苷, BCIG, X-Gal

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About This Item

经验公式（希尔记法）:
C14H15BrClNO6
CAS号:
7240-90-6
分子量:
408.63
Beilstein:
1402009
EC號碼:
230-640-8
MDL號碼:
MFCD00005666
分類程式碼代碼:
12352204
PubChem物質ID:
57653968
NACRES:
NA.83

product name

5-溴-4-氯-3-吲哚基-β-吡咯-D-半乳糖苷, ≥98%

品質等級

200

化驗

≥98%

形狀

powder

溶解度

DMF: methanol (1:1): soluble 50 mg/mL

適合性

suitable for identification of lac+ bacterial colonies

儲存溫度

−20°C

SMILES 字串

OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1

InChI 密鑰

OPIFSICVWOWJMJ-AEOCFKNESA-N

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應用

5-溴-4-氯-3-吲哚 β-D-半乳吡喃糖苷已被用于验证Caki-2/LacZ克隆的转导是否成功。它还可用于转化子的蓝白班筛选。
X-Gal是β-半乳糖苷酶的发色底物，可产生丰富的蓝色，与背景相比可以直观地检测到。 X-Gal是lac+基因型重组细菌菌落蓝白斑筛选的首选基质。

重構

储备溶液（100 mg/ml）是通过将 X-Gal 溶解在二甲基甲酰胺中制备的，该溶液应避光储存在 −20 °C。无需灭菌。

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说明
价格

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

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