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Merck

B6024

Sigma-Aldrich

5-溴-4-氯-3-吲哚基-β-吡咯-D-半乳糖苷

tablet, suitable for identification of lac+ bacterial colonies

别名:

5-溴-4-氯-3-吲哚基-β-吡咯-D-半乳糖苷, BCIG, X-Gal

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About This Item

经验公式(希尔记法):
C14H15BrClNO6
CAS号:
分子量:
408.63
Beilstein:
1402009
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
PubChem物質ID:
NACRES:
NA.83

产品名称

5-溴-4-氯-3-吲哚基-β-吡咯-D-半乳糖苷, tablet

形狀

tablet

品質等級

適合性

suitable for identification of lac+ bacterial colonies

儲存溫度

−20°C

SMILES 字串

OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1

InChI 密鑰

OPIFSICVWOWJMJ-AEOCFKNESA-N

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一般說明

5-溴-4-氯-3-吲哚基 β-D-吡喃半乳糖苷,通常称为 X-Gal,是 β-半乳糖苷酶的组织化学底物。

應用

5-溴-4-氯-3-吲哚基 β-D-吡喃半乳糖苷已用于制备 X-Gal 储备液,并将其添加到 LB 琼脂平板中,用于蓝白色筛选转化子。
X-Gal是β-半乳糖苷酶的发色底物,可产生丰富的蓝色,与背景相比可以直观地检测到。 X-Gal是lac+基因型重组细菌菌落蓝白斑筛选的首选基质。

生化/生理作用

X-gal 通常用于转基因活性的原位检测。

數量

每片含 5 mg 底物

重構

通过将 X-Gal 溶解在二甲基甲酰胺中制备储备液(100 mg/ml),应将其储存在 −20°C 并避光。无需灭菌。

相關產品

产品编号
说明
价格

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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