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Merck
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Key Documents

L2542

Sigma-Aldrich

LB 肉汤

Liquid microbial growth medium

别名:

Luria Broth Miller, Luria-Bertani broth, Miller

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About This Item

分類程式碼代碼:
41106200
NACRES:
NA.85

等級

for molecular biology

品質等級

無菌

sterile; 0.2 μm filtered

形狀

liquid

技術

microbiological culture: suitable

pH值

6.5-7.2

應用

microbiology

儲存溫度

room temp

適合性

nonselective for Escherichia coli
nonselective for coliforms

一般說明

Miller’s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. It enables fast growth, improves growth yields for various bacterial species, and can retain the hydrophilic behavior of samples for longer periods.

應用

LBBroth (Miller) is suitable for:

  • non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.
  • selective cultivation when appropriate antibiotics are added.
  • the cultivation of E-coli cells for anti-bacterial assays.

特點和優勢

Miller′s LB liquid provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package size
  • Standard formulation

成分

10g/L Tryptone
10 g/L NaCl
5 g/L Yeast Extract

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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实验方案

General protocols for growth of competent cells in microbial medium.

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