L3397

LB Broth (Luria low salt)

Powder microbial growth medium

• 别名: Luria Broth (Miller’s Modification), Luria-Bertani medium (LB)
• 分類程式碼代碼: 41106200
• NACRES: NA.85

属性

• 等級: for molecular biology
• 品質等級: 200
• 無菌: non-sterile
• 形狀: powder
• 成份: NaCl, 0.5 g/L ; Tryptone, 10 g/L ; Yeast Extract, 5 g/L
• 技術: microbiological culture: suitable
• pH值: 6.8-7.2(1.55% solution)
• 適合性: suitable for molecular biology; nonselective for Escherichia coli; nonselective for coliforms
• 應用: food and beverages; microbiology
• 儲存溫度: room temp

说明

一般說明

Luria Broth is a bacterial culture medium used for the cultivation of members of the Enterobacteriaceae especially E.coli, as well as for coliphage plaque assays. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a very low-salt formulation.

應用

LB Broth (Luria low salt) has been used as a liquid broth medium in the broth microdilution technique.

特點和優勢

Luria′s low salt LB powder provides:

• Standard formulation
• A budget-friendly alternative to liquid
• Easy scale-up using larger package sizes

準備報告

1. Suspend 15.5 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121°C.

重構

Stir to suspend 15.5g powder in 1L water. Autoclave for 15 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable