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一般說明
核糖核酸酶抑制剂通过形成紧密非共价的1:1复合物抑制RNase活性。 它来源于大肠杆菌,大肠杆菌表达人胎盘核糖核酸酶的部分结构。 抑制RNA酶A、B和C。不抑制RNA酶H、1、T1、S1核酸酶、SP6、T7 RNA聚合酶、T3 RNA聚合酶、AMV逆转录酶、M-MLV逆转录酶或Taq聚合酶。 该抑制剂可通过苯酚萃取或在65°C 下加热10分钟去除。抑制的pH范围是 pH 5.5–9 (pH 7–8之间抑制能力最高)。
應用
核糖核酸酶抑制剂适用于核糖核酸酶的体外 抑制,包括以下应用如:
- 从mRNA合成cDNA
- 逆转录聚合酶链反应(RT-PCR)
- 体外 转录和翻译
- mRNA 降解的保护
- 甲醛交联lncRNA 纯化
- RNA荧光原位杂交(FISH)
适于体外抑制核糖核酸酶,包括cDNA合成、RT-PCR以及体外转录和翻译等步骤。
生化/生理作用
通过形成紧密非共价的 1:1 复合物抑制 RNase。
成分
核糖核酸酶抑制剂,含20 mM HEPES-KOH(pH 7.6)、50 mM KCl、8 mM DTT和50%甘油。
單位定義
一个单位的抑制剂可将一个胞苷2′:3′-环单磷酸盐体系中5ng核糖核酸酶A的活性降低50%。
外觀
溶于 20 mM HEPES-KOH (pH 7.6)、50 mM KCl、8 mM DTT 和 50% 甘油中。
其他說明
- 应当避免变性条件(即脲或温度≥50 °C),因为它们会导致活性核糖核酸酶从复合物上释放。
- 该产品仅用于研发,不用于药物、家庭或其他用途。
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 2
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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