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品質等級
形狀
liquid
用途
sufficient for 1250 reactions
sufficient for 250 reactions
sufficient for 5000 reactions
特點
Difficult Templates/Specialty Enzymes PCR
dNTPs included
hotstart
儲存條件
protect from light
技術
qPCR: suitable
顏色
light blue
輸入
crude DNA
相容性
ABI 5700
ABI 7000
ABI 7300
ABI 7700
ABI 7900 HT
ABI 7900 HT Fast
ABI 7900
ABI StepOnePlus
檢測方法
probe-based
運輸包裝
dry ice
儲存溫度
−20°C
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一般說明
Inhibitor Resistant Genotyping PCR Readymix is a 2X concentrated ready-to-use PCR master mix used for amplification of DNA templates that overcomes inhibitors often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust PCR reagent that provides highly specific and efficient amplification for genotyping sequence variants including allelic discrimination of single nucleotide polymorphisms (SNPs) using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. Inhibitor Resistant Genotyping PCR Readymix contains all required reaction components, except primers, probes, and DNA template. The light blue color of the inert tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with reporter dye fluorescence or affect the stability of the product.
A key component of Inhibitor Resistant Genotyping PCR ReadyMix is an ultra-pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This proprietary polymerase mix is highly resistant to PCR inhibitors and provides an extremely stringent automatic hot-start allowing reaction assembly, and temporary storage, at room temperature prior to PCR amplification. Inhibitor Resistant Genotyping PCR Readymix is compatible with both fast and conventional PCR cycling protocols.
A key component of Inhibitor Resistant Genotyping PCR ReadyMix is an ultra-pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This proprietary polymerase mix is highly resistant to PCR inhibitors and provides an extremely stringent automatic hot-start allowing reaction assembly, and temporary storage, at room temperature prior to PCR amplification. Inhibitor Resistant Genotyping PCR Readymix is compatible with both fast and conventional PCR cycling protocols.
特點和優勢
- 仅需33分钟即可获得检测结果
- 高效和高灵敏度的实时PCR结果
- 极少/无需优化
成分
2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, ROX reference dye, insert blue qPCR dye, and stabilizers.
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume
法律資訊
ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
TaqMan is a registered trademark of Roche Molecular Systems, Inc.
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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