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生物源
Staphylococcus aureus
品質等級
形狀
aqueous ethanol suspension
分析物化學類別
proteins (Immunoglobulins of various mammalian species)
標籤範圍
~6 mg per mL
技術
affinity chromatography: suitable
基質
Sepharose 4B Fast Flow
基質活化
cyanogen bromide
基質結合
amino
基質墊片
1 atom
容量
≥30 mg/mL binding capacity (human IgG)
儲存溫度
2-8°C
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一般說明
Protein A-Sepharose™已用于制定蛋白质相互作用研究策略、改进乳糜泻的检测，以及研究儿童糖尿病。
應用
来自金黄色葡萄球菌的Protein A-Sepharose™ Fast Flow已用于：
- i免疫共沉淀检测
- 免疫耗竭
- 从人血清和血浆中纯化IgG
蛋白A-琼脂糖凝胶用于亲和层析、抗体纯化和表征、免疫亲和基质、蛋白层析、蛋白A、G和L树脂以及重组蛋白表达和分析。蛋白A-琼脂糖凝胶™已用于制定研究蛋白相互作用的策略，改善乳糜泻检测，以及研究儿童糖尿病。
外觀
20% 乙醇混悬液
法律資訊
Sepharose is a trademark of Cytiva
訊號詞
Warning
危險聲明
危險分類
Flam. Liq. 3
水污染物質分類（WGK）
WGK 3
C Macaulay et al.
The Journal of biological chemistry, 270(1), 254-262 (1995-01-06)
During each cell cycle, the nucleus of higher eukaryotes undergoes a dramatic assembly and disassembly. These events can be faithfully reproduced in vitro using cell-free extracts derived from Xenopus eggs. Such extracts contain three major N-acetylglucosaminylated proteins, p200, p97, and
János Roszik et al.
European journal of immunology, 41(5), 1288-1297 (2011-04-07)
T-cell receptors (TCRs) can be genetically modified to improve gene-engineered T-cell responses, a strategy considered critical for the success of clinical TCR gene therapy to treat cancers. TCR:ζ, which is a heterodimer of TCRα and β chains each coupled to
J Ziebuhr et al.
The Journal of biological chemistry, 276(35), 33220-33232 (2001-06-30)
The largest replicative protein of coronaviruses is known as p195 in the avian infectious bronchitis virus (IBV) and p210 (p240) in the mouse hepatitis virus. It is autocatalytically released from the precursors pp1a and pp1ab by one zinc finger-containing papain-like
Mazhar Adli et al.
Nature protocols, 6(10), 1656-1668 (2011-10-01)
Chromatin immunoprecipitation (ChIP) combined with high-throughput sequencing (ChIP-seq) has become the gold standard for whole-genome mapping of protein-DNA interactions. However, conventional ChIP protocols necessitate the use of large numbers of cells, and library preparation steps associated with current high-throughput sequencing
H Sternlicht et al.
Proceedings of the National Academy of Sciences of the United States of America, 90(20), 9422-9426 (1993-10-15)
A role in folding newly translated cytoskeletal proteins in the cytosol of eukaryotes has been proposed for t-complex polypeptide 1 (TCP1). In this study, we investigated tubulin and actin biogenesis in Chinese hamster ovary (CHO) cells. When extracts of pulse-labeled
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