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Merck

P8996

Sigma-Aldrich

Monoclonal Anti-PINCH-1 antibody produced in mouse

clone PINCH-C58, purified immunoglobulin, buffered aqueous solution

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

mouse

品質等級

共軛

unconjugated

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

PINCH-C58, monoclonal

形狀

buffered aqueous solution

分子量

antigen ~37 kDa

物種活性

hamster, bovine, mouse, monkey, canine, human, rat

技術

immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1-2 μg/mL using total cell extract from 293T cells

同型

IgG1

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

human ... LIMS1(3987)
mouse ... Lims1(110829)
rat ... Gcc2(309798)

一般說明

Monoclonal Anti-PINCH-1 (mouse IgG1 isotype) is derived from the hybridoma PINCH-C58 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a peptide of human PINCH1, conjugated to KLH. LIM and senescent cell antigen-like-containing domain protein 1 (LIMS1) or particularly interesting new Cys-His protein 1 (PINCH-1) is widely expressed. It has five LIM domains. It has leucine-rich nuclear export signal (NES) and nuclear localization signal (NLS) at the C-terminus. Human PINCH-1 and 2 are encoded by two different genes that are 82% identical in their amino acid sequence.

免疫原

peptide corresponding to amino acids 281-299 of human PINCH-1, conjugated to KLH.

應用

Monoclonal Anti-PINCH-1 antibody produced in mouse has been used in:
  • enzyme linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunoprecipitation
  • co-immunoprecipitation

生化/生理作用

The interaction with PINCH proteins (PINCH-1 and 2) is important for localization of integrin linked kinase protein to cell matrix contact sites. It plays a key role in cell−matrix adhesion. PINCH2 is co-expressed and co-localized with PINCH-1/ILK complex. PINCH1 interacts with Wilms tumor 1 protein (WT1) in podocytes. Elevated expression of PINCH1 is observed in human laryngeal carcinoma.

外觀

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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分析证书(COA)

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访问文档库

PINCH1 is transcriptional regulator in podocytes that interacts with WT1 and represses podocalyxin expression
Wang D, et al.
Testing, 6(2), e17048-e17048 (2011)
High PINCH1 Expression in Human Laryngeal Carcinoma Associates with Poor Prognosis
Tsinias G, et al.
Analytical Cellular Pathology, 2018 (2018)
PINCH: More than just an adaptor protein in cellular response
Kovalevich J, et al.
Journal of Cellular Physiology, 226(4), 940-947 (2011)
Characterization of PINCH-2, a new focal adhesion protein that regulates the PINCH-1-ILK interaction, cell spreading, and migration
Zhang Y, et al.
The Journal of biological chemistry, 277(41), 38328-38338 (2002)
Iveta Dobreva et al.
Journal of proteome research, 7(4), 1740-1749 (2008-03-11)
Protein-protein interactions play an essential role in the regulation of vital biological functions. Through a network of interactions, integrin-linked kinase (ILK) functions downstream of integrin receptors to control cell spreading, migration, growth, survival, and cell cycle progression. Despite many reports

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