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E1270

Sigma-Aldrich

ECM 凝胶 来源于 Engelbreth-Holm-Swarm 小鼠肉瘤

liquid, BioReagent, suitable for cell culture

别名:

EHS基质

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About This Item

MDL號碼:
MFCD00283131
分類程式碼代碼:
12352207
NACRES:
NA.75

產品線

BioReagent

品質等級

300

形狀

liquid

濃度

8-12 mg/mL

技術

cell culture | mammalian: suitable

UniProt登錄號

P02468
P02469
P10493
P19137
P43407
Q61001
Q61092
Q61789

運輸包裝

dry ice

儲存溫度

−20°C

基因資訊

mouse ... Lama1(16772) , Lama3(16774) , Lama5(16776) , Lamb1-1(16777) , Lamc1(226519) , Lamc2(16782) , Nid1(18073) , Sdc2(15529)

相关类别

應用

ECM凝胶可用于上皮细胞、内皮细胞、肌肉细胞、神经细胞和肿瘤细胞。

生化／生理作用

当达到20-40°C时，ECM基质胶将发生热活化聚合，形成重组基底膜。 当PC12细胞在该ECM胶薄层上生长时，将在2天内形成神经突。

用于生产本品的每个小鼠种群都定期筛查病原体。经检测，LDEV呈阴性。

成分

ECM制剂的蛋白质浓度为8-12 mg/mL（DMEM溶液），主要成分为层粘连蛋白，还含有IV型胶原蛋白、蛋白多糖硫酸乙酰肝素、巢蛋白等其他次要成分。向ECM凝胶中添加IV型胶原蛋白可增加其聚合度。

本品含有50 mg/L硫酸庆大霉素。

注意

如需长期储存，请将本品置于-20°C储存。ECM凝胶可在2-8℃下储存最长72小时。

準備報告

该产品是从小鼠Engelbreth-Holm-Swarm肉瘤中提取的；并经过氯仿透析。使用前，将凝胶置于2-8℃下解冻过夜，并使用预冷至2-8℃的板和移液器将凝胶分配至多孔板。也可使用2-8℃的Dulbecco改良Eagle培养基将凝胶稀释最多两倍，应先稀释、再分配至多孔板。 该产品在20°C下放置5分钟即可成胶。对于长时间操作，应在低于10°C的温度下进行。细胞可接种于0.5 mm厚的凝胶层上或在1mm厚的凝胶层内培养。 如果在凝胶层内培养细胞，请在接种前将细胞加至凝胶中，建议密度为3-4 x 104 个细胞/mL。 可使用溶剂为PBS，不含钙、镁或EDTA且浓度为0.6-2.4单位/mL的蛋白酶，将细胞从凝胶中分离出来。

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产品编号
说明
价格

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析证书(COA)

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