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NA2110

Sigma-Aldrich

GenElute™ 细菌基因组DNA试剂盒

sufficient for 70 purifications

别名:

细菌基因组DNA, Gen Elute

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About This Item

分類程式碼代碼:
41105501
NACRES:
NA.55

用途

sufficient for 70 purifications

品質等級

200

技術

DNA purification: suitable

儲存溫度

15-25°C

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相关类别

一般說明

GenElute 细菌基因组DNA试剂盒可以简单、方便地从革兰氏阴性菌中分离高纯度基因组DNA。对于大多数革兰氏阳性菌，本试剂盒必须结合选购的溶菌酶 (L4919)来有效裂解较厚的肽聚糖细胞壁。为方便您制备溶菌酶储液，我们提供了一种稀释的革兰氏阳性溶解溶液。

该试剂盒将硅胶膜系统的优势与微量离心形式结合在一起，同时无需采用昂贵的树脂、乙醇沉淀，以及诸如苯酚和氯仿等有害化学成分。

應用

纯化所得的细菌基因组DNA可用于以下游应用：
  • 限制性内切酶酶切
  • PCR
  • Southern印迹
  • 克隆

特點和優勢

  • 典型DNA得率：15 μg - 20 μg
  • 提供有适用于革兰氏阳性菌和革兰氏阴性菌的实验方案
  • 可在2小时内获得优质基因组DNA
  • 可纯化出A260/A280 比率介于1.6-1.9之间的DNA
  • 起始材料：不超过1.5 mL培养物
  • 预期得率：至多20 μg
  • 洗脱体积：400 μl
  • 所需时间：70 - 120分钟
  • A260/A280比率：1.6 - 1.9
  • 无需苯酚、氯仿或乙醇沉淀步骤

原則

细菌首先在含有离液盐的溶液之中裂解，以确保大分子彻底变性。之后加入乙醇，从而使DNA在溶解物通过微量离心管在硅胶膜上离心时，结合到膜上。在洗涤去除污染物后，将DNA放在200 μL的Tris-EDTA溶液中洗脱。

预期的基因组DNA得率取决于细菌培养物的细胞密度、细菌种属以及所用的菌株。通过GenElute试剂盒纯化所得的DNA的A260/A280比率介于1.6-1.9之间，最大长度可达50 kb。

其他說明

更多信息，请见www.sigma-aldrich.com/genomicdna。

法律資訊

GenElute is a trademark of Sigma-Aldrich Co. LLC

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • W0263Wash Solution 1化学品安全说明书
  • C2112Column Preparation Solution化学品安全说明书
  • P2308Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology化学品安全说明书
  • R6148RNase A solution化学品安全说明书

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訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Central nervous system, Respiratory system

儲存類別代碼

3 - Flammable liquids

閃點（°F）

77.0 °F - closed cup

閃點（°C）

25 °C - closed cup

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLCM1811
SLCL7908
SLCL7909
SLCL7906
SLCH5588

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