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應用
In order to study the role of gut microbiota in human health and disease, accurate and reproducible microbial data must be obtained.The crucial step in this process is to apply an appropriate methodology to extract microbial DNA. Setting up the optimal DNA isolation procedure is critical for robustness and reproducibility of the results. Inaccurate DNA extraction may result in microbial misrepresentation.
The Microbiome DNA Purification Kit provides a convenient and rapid method to isolate high-molecular weight, high-quality and high yield microbial DNA from stool samples. The kit contains DNA-free reagents to assure that no external contamination is present. The kit uses a combination of chemical, mechanical and thermal lysis with a simple and rapid spin column procedure to purify the DNA. The purified DNA is high molecular weight, high quality and is fully compatible with downstream applications, such as PCR and NGS.
application note: Typical DNA recovery is 5–25μg from 0.2 g of human stool or 0.1 g of mouse stool.
The Microbiome DNA Purification Kit provides a convenient and rapid method to isolate high-molecular weight, high-quality and high yield microbial DNA from stool samples. The kit contains DNA-free reagents to assure that no external contamination is present. The kit uses a combination of chemical, mechanical and thermal lysis with a simple and rapid spin column procedure to purify the DNA. The purified DNA is high molecular weight, high quality and is fully compatible with downstream applications, such as PCR and NGS.
application note: Typical DNA recovery is 5–25μg from 0.2 g of human stool or 0.1 g of mouse stool.
特點和優勢
- Accurate and reproducible results
- Convenient and rapid spin column procedure
- Highly efficient lysis: combination of chemical, mechanical and thermal methods
- High molecular weight, high quality and high yield DNA is obtained
- DNA free reagents to assure no external contamination
- Fully compatible with downstream applications such as PCR, sequencing and NGS
成分
This kit contains reagents sufficient for 50 DNA extractions.
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See datasheet for more information
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- This product is comprised of the following:
- M1 Lysis Buffer MBD5001
- M2 Lysis Boost Buffer MBD5002
- M3 Cleaning Buffer MBD003
- M4 Binding Buffer MBD5004
- M5 Wash Buffer MBD005
- M6 Elution Buffer MBD006
- Columns MBD5007
- Collection Tubes MBD5008
- Lysis Beads MBD5009
See datasheet for more information
法律資訊
GenElute is a trademark of Sigma-Aldrich Co. LLC
訊號詞
Danger
危險分類
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C
安全危害
儲存類別代碼
8A - Combustible corrosive hazardous materials
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