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NA2010

Sigma-Aldrich

GenElute™ 血液基因组DNA试剂盒

sufficient for 70 purifications

别名:

血液基因组DNA, Gen Elute

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About This Item

分類程式碼代碼:
41105501
NACRES:
NA.55

用途

sufficient for 70 purifications

品質等級

200

技術

DNA purification: suitable

儲存溫度

15-25°C
room temp

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相关类别

一般說明

GenElute血液基因组DNA试剂盒可以简单、方便地从新鲜或长时间存放（超过24小时）的全血中分离高纯度基因组DNA。该试剂盒将硅胶膜技术的优势与微量离心形式结合在一起，同时无需采用昂贵的树脂、乙醇沉淀，以及诸如苯酚和氯仿等有害化学成分。

應用

GenElute™血液基因组DNA试剂盒已被用于：
  • 从切除输精管的兔子体内获得的大颗粒（LG）和小颗粒（SG）样品中提取DNA
  • 从血液样本中分离DNA
  • 从剩余血细胞中提取全血DNA

纯化所得的基因组DNA可用于以下下游应用：
  • 限制性内切酶酶切
  • PCR
  • Southern印迹
  • 测序反应
  • 克隆

特點和優勢

  • 起始材料：不超过200 μl新鲜或长时间存放的血液
  • 预期得率：至多10 μg
  • 洗脱体积：400 μl
  • 所需时间：<40分钟
  • A260/A280比率：1.6 - 1.9
  • 兼容多种抗凝血素、包括EDTA、肝素和柠檬酸钠
  • 通常得率为4-10 μg/200 μL全血样本
  • 可获得A260/A280 比率介于1.6-1.9之间的高纯度基因组DNA
  • 可分离长度不超过50 kb的DNA

原則

起始材料首先溶解在含有离液盐的溶液之中，以确保大分子彻底变性。之后加入乙醇，从而使DNA在溶解物通过微量离心管在硅胶膜上离心时，结合到膜上。随附一份Prewash Solution，可帮助与全血样本长时间存放（超过24小时）相关的污染物。在洗涤去除污染物后，将DNA放在200 mL的Tris-EDTA溶液中洗脱。

预期基因组DNA得率取决于所用的起始材料的数量和性质（例如，从200 μl 新鲜的全血之中，可在一小时内分离出4至10 μg RNase A处理的DNA）。通过本试剂盒纯化所得的DNA的A260/A280比率介于1.6-1.9之间，最大长度可达50 kb。

其他說明

更多信息，请见www.sigma-aldrich.com/genomicdna。

法律資訊

GenElute is a trademark of Sigma-Aldrich Co. LLC

试剂盒组分也可单独购买

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说明
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  • R6148RNase A solution化学品安全说明书

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訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

10 - Combustible liquids

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLCL5155
SLCJ7933
SLCF4479
SLCF4478
SLCC6972

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