跳转至内容
Merck
所有图片(4)

主要文件

查看所有文档

NA2010

Sigma-Aldrich

GenElute™ 血液基因组DNA试剂盒

sufficient for 70 purifications

别名:

血液基因组DNA, Gen Elute

登录查看公司和协议定价
所有图片(4)

About This Item

分類程式碼代碼:
41105501
NACRES:
NA.55

用途

sufficient for 70 purifications

技術

DNA purification: suitable

儲存溫度

15-25°C
room temp

正在寻找类似产品? 访问 产品对比指南

相关类别

一般說明

GenElute血液基因组DNA试剂盒可以简单、方便地从新鲜或长时间存放（超过24小时）的全血中分离高纯度基因组DNA。该试剂盒将硅胶膜技术的优势与微量离心形式结合在一起，同时无需采用昂贵的树脂、乙醇沉淀，以及诸如苯酚和氯仿等有害化学成分。

應用

GenElute™血液基因组DNA试剂盒已被用于：
  • 从切除输精管的兔子体内获得的大颗粒（LG）和小颗粒（SG）样品中提取DNA
  • 从血液样本中分离DNA
  • 从剩余血细胞中提取全血DNA

纯化所得的基因组DNA可用于以下下游应用：
  • 限制性内切酶酶切
  • PCR
  • Southern印迹
  • 测序反应
  • 克隆

特點和優勢

  • 起始材料：不超过200 μl新鲜或长时间存放的血液
  • 预期得率：至多10 μg
  • 洗脱体积：400 μl
  • 所需时间：<40分钟
  • A260/A280比率：1.6 - 1.9
  • 兼容多种抗凝血素、包括EDTA、肝素和柠檬酸钠
  • 通常得率为4-10 μg/200 μL全血样本
  • 可获得A260/A280 比率介于1.6-1.9之间的高纯度基因组DNA
  • 可分离长度不超过50 kb的DNA

原則

起始材料首先溶解在含有离液盐的溶液之中，以确保大分子彻底变性。之后加入乙醇，从而使DNA在溶解物通过微量离心管在硅胶膜上离心时，结合到膜上。随附一份Prewash Solution，可帮助与全血样本长时间存放（超过24小时）相关的污染物。在洗涤去除污染物后，将DNA放在200 mL的Tris-EDTA溶液中洗脱。

预期基因组DNA得率取决于所用的起始材料的数量和性质（例如，从200 μl 新鲜的全血之中，可在一小时内分离出4至10 μg RNase A处理的DNA）。通过本试剂盒纯化所得的DNA的A260/A280比率介于1.6-1.9之间，最大长度可达50 kb。

其他說明

更多信息，请见www.sigma-aldrich.com/genomicdna。

法律資訊

GenElute is a trademark of Sigma-Aldrich Co. LLC

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • C2112Column Preparation Solution化学品安全说明书
  • P2308Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology化学品安全说明书
  • R6148RNase A solution化学品安全说明书

推薦

产品编号
说明
价格

相關產品

产品编号
说明
价格

P2308

蛋白酶 K 来源于林伯氏白色念球菌, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology

E1510

溴化乙啶 溶液, BioReagent, for molecular biology, 10 mg/mL in H2O

A9539

琼脂糖, BioReagent, for molecular biology, low EEO

L3287

Escort ™ IV 转染试剂, Lipid reagent for transient and stable transfection of mammalian and insect cells.

T6400

Tris-硼酸-EDTA缓冲溶液, 5× Concentrate

D9780

Lambda DNA Hind III 消化, for DNA electrophoresis

R6148

RNase A 溶液

NA2020

GenElute™ 血液基因组DNA试剂盒, sufficient for 350 purifications

C2112

纯化柱制备液

93221-001

RNAstable®, Tube Kit, for room temp preservation of RNA

T0956

Sigma Universal Transfection Reagent, Polymer reagent for transfecting common mammalian cell lines

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

10 - Combustible liquids

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLCL5155
SLCJ7933
SLCF4479
SLCF4478
SLCC6972

没有发现合适的版本？

如果您需要特殊版本，可通过批号或批次号查找具体证书。

搜索COA

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

其他客户在看

Slide 1 of 1

1 of 1

LookOut &#174; 支原体 PCR 检测试剂盒 Optimized for use with JumpStart&#8482; Taq DNA Polymerase, D9307.

Sigma-Aldrich

MP0035

LookOut ® 支原体 PCR 检测试剂盒

Involvement of macrophage migration inhibitory factor in the pathogenesis of idiopathic orbital inflammatory pseudotumor
Zhao P, et al.
International Journal of Clinical and Experimental Pathology, 9(7), 6659-6671 (2016)
Xiangzhong Zhao et al.
Scientific reports, 6, 33920-33920 (2016-09-27)
Familial renal glycosuria (FRG) is caused by mutations in the SLC5A2 gene, which codes for Na+-glucose co-transporters 2 (SGLT2). The aim of this study was to analyze and identify the mutations in 16 patients from 8 families with FRG. All
Yanxia Gao et al.
Renal failure, 36(8), 1226-1232 (2014-07-01)
The objective of this study is to identify ATP6V1B1, ATP6V0A4 and SLC4A1 genes mutations and assess audiologic characteristics in six Chinese children with primary distal renal tubular acidosis from four unrelated families between the ages of 2 and 13 years.
Umm-e-Kalsoom et al.
Human genetics, 131(3), 415-422 (2011-08-31)
Postaxial Polydactyly (PAP) is characterized by fifth digit duplication in hands and/or feet. Two types of PAP including PAP-A, representing the development of well-formed extra digit, and PAP-B, representing the presence of rudimentary fifth digit, have been described. Both isolated
Sarit S Pattanaik et al.
Acta tropica, 123(2), 136-138 (2012-04-21)
Severe falciparum malaria is a major health problem in Odisha, India, contributing to high mortality. Multi organ dysfunction is a predominant manifestation of severe disease in Odisha, unlike in Africa, where cerebral malaria and anaemia are common. There are several
查看所有相关文献

实验方案

Whole Genome Amplification from Serum or Plasma Protocol

Whole genome amplification (WGA) of plasma and serum DNA presents a unique challenge due to the small amount of nucleic acid in such samples.

Extraction & Amplification of Whole Blood Using WGA-Protocol

Whole blood is a common source of material used to perform genetic analysis. Many times genomic DNA isolated from whole blood samples is of low yield. This protocol is a simple method to isolate DNA from fresh or aged whole blood products. Once the DNA is isolated, it can be amplified using the GenomePlex® Whole Genome Amplification protocol.

GenElute™ Blood Genomic DNA Kit Protocol

The GenElute Blood Genomic DNA Kit Protocol provides a simple and convenient way to isolate pure genomic DNA from fresh or aged whole blood.

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门