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N3254

Sigma-Aldrich

4-硝基苯磷酸盐 二(三羟甲基氨基甲烷) 盐

phosphatase substrate, ≥97% (enzymatic), powder

别名:

4-硝基苯磷酸盐 双(三羟甲基氨基甲烷) 盐

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About This Item

线性分子式:
(C6 H4 N O6 P)( C4 H12 N O3)2
CAS号:
68189-42-4
分子量:
461.36
Beilstein:
1578142
EC號碼:
269-198-6
MDL號碼:
MFCD00037009
分類程式碼代碼:
12352204
PubChem物質ID:
24897633
NACRES:
NA.83

product name

4-硝基苯磷酸盐 二(三羟甲基氨基甲烷) 盐, phosphatase substrate

品質等級

100

化驗

≥97% (enzymatic)

形狀

powder

溶解度

water: 50 mg/mL, clear, colorless to light yellow

儲存溫度

−20°C

SMILES 字串

NC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O

InChI

1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2

InChI 密鑰

XXAXKCWOTRABOW-UHFFFAOYSA-N

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應用

4-硝基苯磷酸盐双(三羟甲基氨基甲烷)可用于：
  • 作为底物定量分析细胞裂解液中的抗酒石酸酸性磷酸酶(TRAP)活性
  • 天然聚丙烯酰胺凝胶电泳(PAGE)测定磷酸酶活性
  • 在酶联免疫吸附测定(ELISA)中用于碱性磷酸酶

生化／生理作用

对硝基苯磷酸(pNPP)具有高灵敏性，在酶联免疫吸附测定(ELISA)中特别适合作为碱性磷酸酶作用底物。该底物反应生成可溶性黄色终产物，可在405nm处通过分光光度法读取。

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

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Enzyme-linked Immunosorbent Assay (ELISA): From A to Z null
Mary E Wood et al.
Vaccine, 36(50), 7737-7743 (2018-11-12)
Chronic wasting disease (CWD) is a fatal prion disease affecting multiple cervid species. Effective management tools for this disease, particularly in free-ranging populations, are currently limited. We evaluated a novel CWD vaccine in elk (Cervus canadensis) naturally exposed to CWD
Ellen K Wasan et al.
Vaccine, 37(11), 1503-1515 (2019-02-12)
We previously developed an highly efficacious combination adjuvant comprised of innate defense regulator (IDR)-1002 peptide, poly(I:C) and polyphosphazene (TriAdj). Here we aimed to design and test the in vivo efficacy of a mucoadhesive nasal formulation of this adjuvant. To determine
Karol Bomsztyk et al.
Nucleic acids research, 47(12), e69-e69 (2019-03-31)
Chromatin immunoprecipitation (ChIP) is the most widely used approach for identification of genome-associated proteins and their modifications. We have previously introduced a microplate-based ChIP platform, Matrix ChIP, where the entire ChIP procedure is done on the same plate without sample

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Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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