N3129

4-硝基苯磷酸盐 双环己铵盐

phosphatase substrate, chromogenic, ≥97% anhydrous basis, powder

• 别名: p-Nitrophenyl phosphate bis(cyclohexylammonium) salt, PNPP
• 线性分子式: O₂NC₆H₄OP(O)(O^-)₂ · 2C₆H₁₁NH₃⁺
• CAS号: 52483-84-8
• 分子量: 417.44
• Beilstein: 6553276
• EC號碼: 257-949-0
• MDL號碼: MFCD00013173
• 分類程式碼代碼: 12352204
• PubChem物質ID: 24897623
• NACRES: NA.83

属性

• 产品名称: 4-硝基苯磷酸盐 双环己铵盐, phosphatase substrate
• 品質等級: 300
• 化驗: ≥97% anhydrous basis
• 形狀: powder
• 溶解度: water: 10 mg/mL, clear, colorless to faintly yellow
• 儲存溫度: −20°C
• SMILES 字串: NC1CCCCC1.NC2CCCCC2.OP(O)(=O)Oc3ccc(cc3)[N+]([O-])=O
• InChI: 1S/C6H6NO6P.2C6H13N/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*7-6-4-2-1-3-5-6/h1-4H,(H2,10,11,12);2*6H,1-5,7H2
• InChI 密鑰: KXNNUYCXODNFBD-UHFFFAOYSA-N

说明

應用

4-Nitrophenyl phosphate bis(cyclohexylammonium) salt has been used as a component in the colorimetric reagent for abscisic acid (ABA) assay.

生化／生理作用

p-Nitrophenyl phosphate (pNPP) is the preferred substrate for use with alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures. Due to its high sensitivity, pNPP is the considered substrate in alkaline phosphatase systems. ELISA applications based on pNPP can be read in timed assays or delayed readings by stopping with alkaline solutions. This substrate yields a yellow-colored soluble end product that can be measured spectrophotometrically at 405 nm.

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, type N95 (US)