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品質等級
輸入
protein
檢測方法
colorimetric
相關疾病
cancer; hematological disorder; gastrointestinal diseases; neurological disorders; cardiovascular diseases
儲存溫度
−20°C
一般說明
Para-nitrophenyl phosphate (pNPP) is a chromogenic substrate for most phosphatases such as alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases. The reaction yields para-nitrophenol, which becomes an intense yellow soluble product under alkaline conditions and can be conveniently measured at A405 nm on a spectrophotometer.
應用
The pNPP Phosphatase Assay Kit may be used for:
- Metabolism Research
- Cardiovascular Research
- Neurodegenerative Research
特點和優勢
Simplified Process: Enjoy a hassle-free procedure that can be completed within 30 minutes without wash or reagent transfer steps, saving you time and effort.
Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.
Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.
適合性
The kit is suitable for the quantitative determination of phosphatase activity of protein phosphatases.
原則
The pNPP Phosphatase Assay kit involves adding a single reagent to the phosphatase and measuring the product formation on an absorbance reader. The assay can be conveniently performed in cuvettes, tubes or multi-well plates at either room temperature or 37 °C.
其他說明
For additional information on our range of Biochemicals, please complete this form.
訊號詞
Danger
危險分類
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
標靶器官
Nervous system,Kidney,Liver,Skin
儲存類別代碼
8A - Combustible corrosive hazardous materials
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