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Merck

MAK446

Sigma-Aldrich

Acid Phosphatase Assay Kit

Sufficient for 100 colorimetric tests

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84

品質等級

輸入

serum
plasma
tissue sample(s)
cell lysate

檢測方法

colorimetric

相關疾病

cancer, prostate tumor; gastrointestinal diseases

儲存溫度

−20°C

一般說明

Acid Phosphatase (ACP) is an enzyme which catalyzes the cleavage of phosphate groups from other molecules during digestion. Acid phosphatase can be found in lysosomes and become active after fusing with endosomes, acidifying the pH and, thus, creating an optimal environment for ACP. ACP can also be found in bone, spleen, liver, kidney and blood. Serum levels can be used as a biomarker for prostatic carcinoma, although prostate-specific antigen (PSA) is more widely used.

應用

The Acid Phosphatase Assay Kit may be used for:
  • Cancer Research
  • Gastrointestinal Research
  • Metabolism Research

特點和優勢

Efficient and Precise: Requires only 20 μL of sample with a 30-minute assay reaction to detect acid phosphatase levels across a broad range from 0.05 to 60 U/L, providing both convenience and sensitivity.

Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

適合性

The kit is suitable for the determination of acid phosphatase in biological samples (e.g. plasma, serum, cell lysate, tissue samples.)

原則

The non-radioactive, colorimetric Acid Phosphatase Assay Kit is based on the cleavage of p-nitrophenol from the synthetic substrate. p-Nitrophenol becomes intensely colored after addition of the stop reagent. The increase in absorbance at A405 nm after addition of the stop reagent is directly proportional to the enzyme activity.

其他說明

For additional information on our range of Biochemicals, please complete this form.

訊號詞

Danger

危險分類

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

標靶器官

Nervous system,Kidney,Liver,Skin

儲存類別代碼

8A - Combustible corrosive hazardous materials


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