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用途
sufficient for 100 colorimetric tests
檢測方法
colorimetric
相關疾病
cancer; orthopedic diseases; aging/geriatric diseases
儲存溫度
−20°C
基因資訊
human ... PFKL(5211) , PFKM(5213) , PFKP(5214)
mouse ... PFKL(18641) , PFKM(18642) , PFKP(56421)
rat ... PFKL(25741) , PFKM(65152) , PFKP(60416)
一般說明
磷酸果糖激酶 (PFK) 催化糖酵解的第三步，即果糖-6-磷酸转化为果糖-1,6-二磷酸，即糖酵解的限速步骤。PFK 活性除转化后修饰外，还受多种辅助因子的高度调控，可用于测量组织中的糖酵解通量。缺乏 PFK 活性可导致糖原贮积病，糖原贮积病类型 II 型（Tarui 病）——一种糖原贮积病。
應用
磷酸果糖激酶 (PFK) 活性比色试剂盒用于测定磷酸果糖激酶的活性。
適合性
适用于测定磷酸果糖激酶活性，分析葡萄糖代谢和细胞信号，筛选动物组织（肝、脑、心脏、肌肉等）和细胞培养（贴壁细胞或悬浮细胞）中的抗癌药物。
原則
磷酸果糖激酶比色测定试剂盒为测定多种样品中的 PFK 活性提供了一种简单直接的程序。通过偶联酶试验测定 PFK 活性，其中果糖-6-磷酸和 ATP 通过 PFK 转化为果糖-1,6-二磷酸和 ADP。ADP 被酶混合物转化为 AMP 和 NADH。生成的 NADH 还原无色探针，生成与 PFK 活性成比例的比色 (450 nm) 产物。PFK 的 1 个单位是 37°C、pH 7.4 时每分钟生成 1.0 μmol NADH 的酶量。
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
Neng Tang et al.
Biochemical and biophysical research communications, 526(1), 1-7 (2020-03-21)
The mechanisms underlying the initiation and proliferation of liver regeneration (LR) has been extensively studied using the partial hepatectomy (PHx) model, while little is known about the termination of LR. PP2Acα (protein phosphatase 2 A catalytic subunit α isoform) is
Manipulation of a glycolytic regulator alters growth and carbon partitioning in the marine diatom Thalassiosira pseudonana.
Abbriano R, et al.
Algal research, 32, 250-258 (2018)
Kai Zhou et al.
Cell death & disease, 9(10), 988-988 (2018-09-27)
Plastic phenotype convention between glioma stem cells (GSCs) and non-stem tumor cells (NSTCs) significantly fuels glioblastoma heterogeneity that causes therapeutic failure. Recent progressions indicate that glucose metabolic reprogramming could drive cell fates. However, the metabolic pattern of GSCs and NSTCs
M Ristow et al.
The Journal of clinical investigation, 100(11), 2833-2841 (1998-02-12)
Non-insulin-dependent diabetes mellitus (NIDDM) is caused by peripheral insulin resistance and impaired beta cell function. Phosphofructo-1-kinase (PFK1) is a rate-limiting enzyme in glycolysis, and its muscle subtype (PFK1-M) deficiency leads to the autosomal recessively inherited glycogenosis type VII Tarui's disease.
T D Howard et al.
Genomics, 34(1), 122-127 (1996-05-15)
Phosphofructokinase (PFK) is a key rate-limiting enzyme in glycolysis and represents a major control point in the metabolism of glucose. There are at least three known isoforms of PFK in humans, referred to as the muscle, platelet, and liver forms
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