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生物源
Escherichia coli (EH100 Ra mutant)
品質等級
形狀
lyophilized powder
雜質
<3% Protein (Lowry)
顏色
white to yellow
溶解度
water: 4.90-5.10 mg/mL, cloudy to turbid, colorless to faintly yellow
運輸包裝
ambient
儲存溫度
2-8°C
一般說明
本品是使用苯酚:氯仿:石油醚从大肠杆菌EH 100(Ra突变株)中提取的。来源菌株由私人提供。
生化/生理作用
脂多糖(LPS)位于无荚膜菌株的膜外层,暴露于细胞表面。它们有助于维持外膜的完整性,并防止胆汁盐和亲脂性抗生素破坏细胞。
準備報告
通过苯酚-氯仿-石油醚萃取进行制备。
本品可溶于水(5 mg/ml)或细胞培养基(1 mg/ml),产生混浊的淡黄色溶液。溶于生理盐水并涡旋和加热至70-80 oC后,可形成浓度更高但仍然混浊的溶液(20 mg/ml)。脂多糖分子可在各种溶剂中形成胶束。溶于水和磷酸盐缓冲盐水时,可观察到浑浊的溶液。使用有机溶剂不能形成更清晰的溶液。使用甲醇可产生具有漂浮物的混浊悬浮液,而溶于水会产生均匀的混浊溶液。
本品可溶于水(5 mg/ml)或细胞培养基(1 mg/ml),产生混浊的淡黄色溶液。溶于生理盐水并涡旋和加热至70-80 oC后,可形成浓度更高但仍然混浊的溶液(20 mg/ml)。脂多糖分子可在各种溶剂中形成胶束。溶于水和磷酸盐缓冲盐水时,可观察到浑浊的溶液。使用有机溶剂不能形成更清晰的溶液。使用甲醇可产生具有漂浮物的混浊悬浮液,而溶于水会产生均匀的混浊溶液。
訊號詞
Danger
危險聲明
危險分類
Acute Tox. 2 Oral
儲存類別代碼
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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