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應用
大豆酶将使用花生四烯酸作为底物,以亚油酸作为底物的活性约为 15%;花生四烯酸氧化的产物是 12-或 15-过氧化氢花生四烯酸(12-HPETE 或 15-HPETE)。
来自Glycine max (大豆)的脂氧酶(也称脂肪氧合酶)已被用于修饰从人血浆中分离的低密度脂蛋白。
生化/生理作用
催化含有顺式,顺式-1,4-戊二烯结构的脂质的氢过氧化。
單位定義
一个酶活性单位是指在pH 9.0、25°C 下,在3.0 ml体积(1 cm光程)中使用亚油酸作为底物时,每分钟使A234 增加0.001所需的酶量。一个A234 单位相当于μmole亚油酸的氧化。
外觀
悬浮于2.3 M(NH4)2SO4溶液中,pH约为6.0
準備報告
通过离子交换色谱和疏水相互作用色谱法制备。
分析報告
通过缩二脲法测定的蛋白质。
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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P Harduin et al.
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Instructions for working with enzymes supplied as ammonium sulfate suspensions
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