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product name
粒细胞巨噬细胞集落刺激因子 人, GM-CSF, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture
生物源
human
品質等級
重組細胞
expressed in HEK 293 cells
化驗
≥95% (SDS-PAGE)
形狀
lyophilized powder
效力
≤5 ng/mL EC50
品質
endotoxin tested
分子量
dimer 15-36 kDa (glycosylated)
包裝
pkg of 5X10 μg
pkg of 10 μg
儲存條件
avoid repeated freeze/thaw cycles
技術
cell culture | mammalian: suitable
雜質
≤1 EU/mg
UniProt登錄號
儲存溫度
−20°C
基因資訊
human ... CSF2(1437)
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一般說明
粒细胞-巨噬细胞集落刺激因子定位于人类染色体5q21-q32。
應用
人类粒细胞-巨噬细胞集落刺激因子已用于人类急性髓系白血病细胞系(OCI-M2)的集落刺激细胞因子,以测试其对5-氮杂胞苷(AZA)介导的上游调控元件(URE) DNA去甲基化的影响。
生化/生理作用
粒细胞-巨噬细胞集落刺激因子(GM-CSF)是一种免疫调节剂和促炎细胞因子。它与粒细胞、内皮细胞和巨噬细胞等免疫细胞中的异质细胞表面受体结合。GM-CSF参与肺和肾系统的炎症相关疾病。
粒细胞-巨噬细胞集落刺激因子(GM-CSF)是一种粒细胞、巨噬细胞和嗜酸性粒细胞谱系中的细胞生长和分化因子。GM-CSF在极低浓度下刺激多潜能祖细胞集落形成,是造血祖细胞在所有分裂过程中存活和增殖的重要因素。 它还能刺激一些上皮细胞和破骨细胞的生长。GM-CSF由多种细胞类型(单核细胞、内皮细胞、T细胞、成纤维细胞、丝裂原刺激的B细胞和脂多糖刺激的巨噬细胞)产生。GM-CSF是一种含有128个氨基酸的单链糖蛋白,采用保守二硫键。 人类和小鼠GM-CSF共享约。序列同源性54%,不发生生物活性交叉反应。
注意
虽然人类和小鼠GM-CSF有54%的氨基酸序列同源性,但它们的生物作用是物种特异性的,未表现跨物种反应性。
外觀
从0.2 μm过滤的 1x PBS溶液中冻干。
準備報告
HumanKine GM-CSF在人HEK 293细胞中表达为15- 36 kda的糖基化单体。在人HEK293细胞中生成可提供真正的糖基化。糖基化有助于细胞生长培养基和其他应用中的稳定性。虽然人类和小鼠GM-CSF有54%的氨基酸序列同源性,但它们的生物作用是物种特异性的,未表现跨物种反应性。
分析報告
通过人 TF-1 细胞(人红白血病指示细胞系)增值的剂量依赖性刺激测定比活性。
法律資訊
HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
其他客户在看
Science (New York, N.Y.), 230(4731), 1282-1285 (1985-12-13)
Human granulocyte-macrophage colony-stimulating factor (GM-CSF) is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts directly on mature neutrophils. A full-length complementary DNA clone encoding human GM-CSF was used as a probe to screen a human
Immunological reviews, 238(1), 76-92 (2010-10-26)
Understanding the diversification of dendritic cell (DC) lineages is one of the last frontiers in mapping the developmental hierarchy of the hematopoietic system. DCs are a vital link between the innate and adaptive immune responses; thus, elucidating their developmental pathways
Leukemia & lymphoma, 50(9), 1412-1421 (2009-07-16)
In autologous stem cell transplantation, mobilized peripheral blood has replaced the bone marrow as the preferred source of hematopoietic stem cells (HSCs). Because HSCs normally exist in the blood in very low numbers, the use of agents to "mobilize" HSCs
International journal of hematology, 100(4), 361-369 (2014-07-25)
Myelodysplastic syndrome (MDS) is a stem cell tumor characterized by dysplastic features and ineffective hematopoiesis in the early phase and leukemic progression in the late phase. Speculating that differences in the expression of genes and microRNA (miRNA) in control and
Blood, 125(20), 3173-3182 (2015-03-15)
Because activating killer cell immunoglobulinlike receptors (KIRs) are heterogeneously expressed in the population, we investigated the role of donor activating KIRs in haploidentical hematopoietic transplants for acute leukemia. Transplants were grouped according to presence vs absence of KIR-ligand mismatches in
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