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Merck

G5035

Sigma-Aldrich

粒细胞巨噬细胞集落刺激因子 人

≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

别名:

GM-CSF 人

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About This Item

MDL號碼:
分類程式碼代碼:
12352202
NACRES:
NA.77

产品名称

粒细胞巨噬细胞集落刺激因子 人, GM-CSF, recombinant, expressed in E. coli, suitable for cell culture

生物源

human

品質等級

重組細胞

expressed in E. coli

化驗

≥98% (SDS-PAGE)

形狀

lyophilized powder

效力

≤0.1 ng/mL EC50

分子量

14.6 kDa

包裝

pkg of 4X5 μg
pkg of 5 μg

儲存條件

avoid repeated freeze/thaw cycles

技術

cell culture | mammalian: suitable

雜質

≤1.000 EU/μg

顏色

white

溶解度

water: soluble 0.010 mL, clear, colorless

UniProt登錄號

儲存溫度

−20°C

基因資訊

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一般說明

基于蛋白质含量的包装尺寸

生化/生理作用

粒细胞-巨噬细胞集落刺激因子(GM-CSF)是一种粒细胞、巨噬细胞和嗜酸性粒细胞谱系中的细胞生长和分化因子。GM-CSF在极低浓度下刺激多潜能祖细胞集落形成,是造血祖细胞在所有分裂过程中存活和增殖的重要因素。 它还能刺激一些上皮细胞和破骨细胞的生长。GM-CSF由多种细胞类型(单核细胞、内皮细胞、T细胞、成纤维细胞、丝裂原刺激的B细胞和脂多糖刺激的巨噬细胞)产生。GM-CSF是一种含有128个氨基酸的单链糖蛋白,采用保守二硫键。 人类和小鼠GM-CSF共享约。序列同源性54%,不发生生物活性交叉反应。

外觀

从 0.2μm 过滤的 10mM 柠檬酸钠溶液 (pH3.5) 中冻干。

分析報告

使用人 TF-1 细胞在培养物中检测人 GM-CSF 的增殖活性。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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International journal of hematology, 100(4), 361-369 (2014-07-25)
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T Kitamura et al.
Journal of cellular physiology, 140(2), 323-334 (1989-08-01)
We have established a novel cell line, designated as TF-1, from a patient with erythroleukemia, which showed complete growth dependency on granulocyte-macrophage colony-stimulating factor (GM-CSF) or on interleukin-3 (IL-3) and carried a homogeneous chromosomal abnormality (54X). Erythropoietin (EPO) also sustained

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