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F0291

Sigma-Aldrich

Fibroblast Growth Factor-Basic, human

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

别名:

成纤维细胞生长因子 7-碱性 人, FGF2, bFGF

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About This Item

CAS号:
106096-93-9
MDL號碼:
MFCD00081382
分類程式碼代碼:
12352202
NACRES:
NA.77

product name

hBFGF, FGF-Basic, recombinant, expressed in E. coli, suitable for cell culture

生物源

human

品質等級

200

重組細胞

expressed in E. coli

化驗

≥97% (SDS-PAGE)

形狀

lyophilized powder

效力

≤-0.6 ng/mL

分子量

16.0 kDa

包裝

pkg of 4X25 μg
pkg of 25 μg

儲存條件

avoid repeated freeze/thaw cycles

技術

cell culture | mammalian: suitable

雜質

≤1.00 EU/μg

顏色

white to faint yellow cast

溶解度

water: soluble 0.025 mg, clear, colorless to faintly yellow

UniProt登錄號

P09038

儲存溫度

−20°C

基因資訊

human ... FGF2 (2257)

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相关类别

一般說明

碱性成纤维细胞生长因子（FGF-Basic） 存在于基底膜和内皮下细胞外基质中。FGF-Basic 在胚胎发育早期被诱导，它是胚胎干细胞（ESC） 和谱系定义的干细胞的多能性和自我更新所必需的（但不充分）； 其中它起到支持增殖和抑制分化的作用。hESC 的自我更新取决于激活素/Nodal/Smad2,3 分支的激活和 TGFβ 家族细胞信号传导程序的 BMP/GDF/Smad5 分支的抑制。FGF-Basic 是多能性节点支持的感受态因子。FGF-Basic 是维持干细胞特性所必需且充分的。它诱导人 ES 细胞中 Nodal 和 FGF-Basic 的表达。

應用

碱性成纤维细胞生长因子（FGF-Basic）是维持培养物中人胚胎干细胞和各种其它干细胞系，如间充质干 （基质） 细胞 （MSC） 所必需的。

外觀

从 20 mM Tris、1 M NaCl（pH 7.0，每1 μg BFGF含 50μg 牛血清白蛋白 (BSA)/1μg 脂联素）的 0.2 μm 过滤溶液冻干。

分析報告

FGF-Basic 的生物活性是在荧光测定中使用氧化还原敏感型染料刃天青进行测量。

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

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Sigma-Aldrich

Sigma-Aldrich

GF003

人重组成纤维细胞生长因子碱性蛋白

C Xu et al.
Nature biotechnology, 19(10), 971-974 (2001-10-03)
Previous studies have shown that maintenance of undifferentiated human embryonic stem (hES) cells requires culture on mouse embryonic fibroblast (MEF) feeders. Here we demonstrate a successful feeder-free hES culture system in which undifferentiated cells can be maintained for at least
R Beklem Bostancioglu et al.
Colloids and surfaces. B, Biointerfaces, 155, 415-428 (2017-05-02)
Accelerated Mesenchymal Stem Cells (MSCs) condensation and robust MSC-matrix and MSC-MSC interactions on nano-surfaces may provide critical factors contributing to such events, likely through the orchestrated signal cascades and cellular events modulated by the extracellular matrix. In this study, human
Chunhui Xu et al.
Stem cells (Dayton, Ohio), 23(3), 315-323 (2005-03-08)
Previous studies have shown that prolonged propagation of undifferentiated human embryonic stem cells (hESCs) requires conditioned medium from mouse embryonic feeders (MEF-CM) as well as matrix components. Because hESCs express growth factor receptors, including those for basic fibroblast growth factor
Lei Xiao et al.
Stem cells (Dayton, Ohio), 24(6), 1476-1486 (2006-02-04)
Human embryonic stem cells (hESCs) self-renew indefinitely while maintaining pluripotency. The molecular mechanism underlying hESCs self-renewal and pluripotency is poorly understood. To identify the signaling pathway molecules that maintain the proliferation of hESCs, we performed a microarray analysis comparing an
Structural characterization and biological functions of fibroblast growth factor.
D Gospodarowicz et al.
Endocrine reviews, 8(2), 95-114 (1987-05-01)
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