推荐产品
特點
wetted part: no
包裝
pkg of 50 ea (columns)
製造商／商標名
Cytiva 28-9180-08
儲存條件
dry media
床尺寸
13 mm × 26 mm
床體積
3.5 mL
柱內徑
13 mm
基質
cross-linked dextran
粒徑
>50 μm (dry)
85-260 μm
cleaning
2-13
工作範圍
2-13
適合性
suitable for bioprocess medium
一般說明
PD MidiTrap G-25 is a convenient and rapid cleanup with high reproducibility and the potential for high throughput by running multiple samples in parallel. Can be used in a centrifugation or gravity protocol.
PD MidiTrap G-25 is designed for convenient desalting and buffer exchange of biological samples and volumes up to 1.0 mL can be handled. Two alternative application protocols are available, gravity or centrifugation. By using the gravity protocol, a simple cleanup of the sample is done with one or several columns in parallel without any need for a purification system. With the centrifugation protocol, the samples are run in parallel in a standard centrifuge with minimal dilution of the eluted sample. Four adapters are included in each product pack to enable easy centrifugation.
PD MidiTrap G-25 is designed for convenient desalting and buffer exchange of biological samples and volumes up to 1.0 mL can be handled. Two alternative application protocols are available, gravity or centrifugation. By using the gravity protocol, a simple cleanup of the sample is done with one or several columns in parallel without any need for a purification system. With the centrifugation protocol, the samples are run in parallel in a standard centrifuge with minimal dilution of the eluted sample. Four adapters are included in each product pack to enable easy centrifugation.
應用
Protein sample preparation. Desalting/Buffer Exchange/Cleanup.
特點和優勢
- Reliable and reproducible cleanup of proteins and other large biomolecules (>5000 Mr).
- Range of applications such as desalting, buffer exchange, and removal of low-molecular weight compounds.
- High desalting capacity.
- Flexible protocols with ability to use gravity flow or centrifugation.
- Sample preparation in the range of 0.5 to 1.0 mL for gravity and 0.75 to 1.0 mL with centrifugation.
儲存和穩定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.15% Kathon CG/ICP Biocide)
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)
訊號詞
Warning
危險聲明
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
商品
This page describes characteristics of Glutathione Sepharose and HiTrap Benzamidine FF (high sub) media and columns from GE Healthcare.
This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.
实验方案
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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