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Merck

10104159001

Roche

DNase I

grade II, from bovine pancreas

别名:

脱氧核糖核酸酶, 脱氧核苷酸酶 5′-寡核苷酸脱氢酶

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About This Item

分類程式碼代碼:
12352204

生物源

bovine pancreas

品質等級

形狀

lyophilized

包裝

pkg of 100 mg

製造商/商標名

Roche

技術

DNA extraction: suitable

最適pH

~7.0

儲存溫度

2-8°C

一般說明

牛胰腺脱氧核糖核酸酶I(DNase I)是DNA小沟相互作用的核酸酶,特异性相对较低。该蛋白质由两个中心β 折叠构成,而每个折叠由六个β-链所组成。该结构被大量的环以及α-螺旋区域包围。该酶与核酸外切酶III具有结构相似性。DNase I是研究得最透彻的哺乳动物来源的内切酶之一。它是一种双链特异性内切核酸酶,需要二价阳离子激发最大活性。

應用

蛋白质(膜蛋白)的分离操作。
牛胰腺脱氧核糖核酸酶I(DNase I)已被用于:
  • 分离肺、皮肤和肿瘤样品中的细胞
  • 从抗DNase I的活细菌细胞中提取细菌DNA †

單位定義

一个单位表示在测定条件下导致吸光度以0.001每分钟的速率而增加的酶活力。

準備報告

激活剂:Ca2+ (0.12 mM)与Mg2+结合在一起可增强活性 [Laskowski (1971); Melgar及Goldthwait (1968)].
工作浓度:1 mg/ml
在溶解过程中请勿涡旋!
储备条件 (工作溶液):当在水中重悬时,溶液可在2至8 °C下保存2至3天。在溶解过程中请勿涡旋。注:至少以1 mg/ml溶解。

其他說明

仅用于生命科学研究。不可用于诊断。

象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Resp. Sens. 1 - Skin Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

does not flash

閃點(°C)

does not flash


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