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品質等級
技術
buffer exchange: suitable
基質活性組
phase
膨潤
1 g swells to 4-6 mL
珠尺寸
50-150 μm
應用
life science and biopharma
相容性
Cytiva
InChI
1S/C6H12O6.C3H8O3/c7-1-2-3(8)4(9)5(10)6(11)12-2;4-1-3(6)2-5/h2-11H,1H2;3-6H,1-2H2/t2-,3-,4-,5-,6-;/m1./s1
InChI 密鑰
BCPVZFFJGCURNR-OCOFDJSDSA-N
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一般說明
Sephadex® G-25葡聚糖凝胶由葡聚糖与环氧氯丙烷交联而成，用作体积排阻和凝胶过滤树脂。不同类型的Sephadex的交联度不同，因此其溶胀程度和分子分离范围也不同。Sephadex G-25是G型Sephadex中的一种型号。G型系列分5种型号，具有不同的分子量分离范围。其中，G-10适合分离小分子，G-75适合大分子。Sephadex G-25保证可靠供应并提供全面的技术和监管支持，以满足工业要求。
應用
Sephadex® G-25葡聚糖凝胶可用于通过凝胶渗透色谱将标记蛋白与未反应染料N-羟基琥珀酰亚胺酯(NHS)分离。
分馏范围（MW）
右旋糖酐：100 - 5,000
球状蛋白质1,000 - 5,000
Sephadex® G-25已用于：
右旋糖酐：100 - 5,000
球状蛋白质1,000 - 5,000
Sephadex® G-25已用于：
- 蛋白酶脱盐
- 在放射性碘标记oTP-1的表征和受体分析确认过程中，用色谱柱从游离碘中分离放射性碘标记的oTP-1
生化／生理作用
Sephadex® G-25葡聚糖凝胶是应用广泛的凝胶过滤树脂，主要用于工业应用中的脱盐和缓冲液置换。亲水基质可最大限度减少非特异性吸附，获得高收率的蛋白和核酸。Sephadex® G-25常用于亲和层析、蛋白质层析和凝胶过滤层析。
特點和優勢
- 一步完成快速脱盐、除污染和置换新缓冲液。
- 高回收率，极低的样品稀释度。
- 提供预装树脂的HiPrep脱盐柱和HiTrap脱盐柱，用于方便快速地脱盐
- 专门设计用作生物工艺树脂，适合工业应用。
法律資訊
Sephadex is a registered trademark of Cytiva
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
K M Yocom et al.
Proceedings of the National Academy of Sciences of the United States of America, 79(22), 7052-7055 (1982-11-01)
A stable complex between pentaammineruthenium(III) and histidine-33 in horse heart ferricytochrome c is formed in the reaction between aquopentaammineruthenium(II) and the protein at pH 7. HPLC of the tryptic hydrolysate of the modified protein was employed to identify the pentaammineruthenium
L R Hale et al.
Genetics, 129(1), 103-117 (1991-09-01)
Preliminary studies with restriction fragment length polymorphisms of mitochondrial DNA (mtDNA) in natural populations of Drosophila melanogaster revealed considerable variation in terms of nucleotide sequence and overall size. In this report we present data from more isofemale lines and more
David R Halpin et al.
PLoS biology, 2(7), E173-E173 (2004-06-29)
Recently reported technologies for DNA-directed organic synthesis and for DNA computing rely on routing DNA populations through complex networks. The reduction of these ideas to practice has been limited by a lack of practical experimental tools. Here we describe a
J J Knickerbocker et al.
Biology of reproduction, 40(2), 361-369 (1989-02-01)
Scatchard analysis was used to determine the distribution, number, and affinity of unoccupied receptors for ovine trophoblast protein-1 (oTP-1) in endometrium of sheep throughout the estrous cycle and early pregnancy. In Experiment I, oTP-1 receptor characteristics were determined in membrane
Characterization of endometrial receptors for ovine trophoblast protein-1 during the estrous cycle and early pregnancy in sheep
Knickerbocker JJ and Niswender GD
Biology of Reproduction, 40(2), 361-369 (1989)
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