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Key Documents

GE27-4570-01

GST Bulk Kit

Cytiva 27-4570-01, pack of 1 kit

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About This Item

分類程式碼代碼:
13111000
NACRES:
NA.56

包裝

pack of 1 kit

製造商/商標名

Cytiva 27-4570-01

運輸包裝

wet ice

儲存溫度

2-8°C

一般說明

GST bulk kit is designed for small-scale manual purification of GST-tagged proteins using either column chromatography or batch method. Facilitates optimization studies of expression, solubility conditions and purification parameters of GST-tagged proteins.

應用

GST Bulk Kit contains a 10 mL bulk pack of Glutathione Sepharose 4B medium, five gravity-fLow columns, IPTG, and GST Buffer Kit. GST-tagged Proteins can be recovered under mild elution conditions (10 mM glutathione), which preserves functionality of the Proteins. The binding capacity of Glutathione Sepharose 4B, which can be greater than 10 mg Protein/mL medium, varies with the nature of the tagged Protein and the exact binding conditions used.

特點和優勢

  • For rapid purification of GST-tagged Proteins using gravity flow columns or using batch purification with high specificity.
  • Preserves Protein antigenicity and function.
  • Yields up to 50 mg of pure Protein.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

Sepharose is a trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


分析证书(COA)

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商品

This page describes troubleshooting strategies in purification of GST-tagged proteins using GE Healthcare products.

实验方案

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from GE Healthcare.

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